Fully laparoscopic pancreaticojejunostomy, Puestow procedure (with video).

Chronic pancreatitis usually requires medical treatment and, in rare cases, surgical intervention. The most frequent cause of consultation is chronic pain that is difficult to manage, and therapeutic options such as endoscopy can resolve most problems without surgery. However, when these options fail, surgical management is necessary.

["Helping ourselves": the Ludwigshafen multi-family training].

Eia Asen developed multi-family-therapy (MFT) in the context of pediatric psychiatric work with predominantly poor and marginalised families from very different cultural, religious, linguistic, and social traditions in inner-city London. This work began with families affected by globalisation, migration, segregation, criminality, and other adverse social and economic effects of Thatcherism. Asen replaced the expert-led diagnostics, patterns of illness and individual medical indication of the case-by-case based model of treatment with a systemic practice developed across decades together with a large multi-professional and multicultural team, which places the family, the social, economic, and educational contexts, as well as traditions and the every-day situations of children and their parents, at the centre of the support.

Protein-solute interactions affect the outcome of ultrafiltration/diafiltration operations.

Protein production operations often involve a final diafiltration of the protein into formulation buffer. For several Amgen product proteins, post-diafiltration assays revealed a significant difference in molar excipient concentrations on the retentate and the permeate side of the membrane. For example, post-diafiltration assays of formulated 200 mg/mL human interleukin-1 receptor antagonist showed molar chloride concentrations up to 30% lower than those of the diafiltration buffer.

ABRF-ESRG'03: analysis of a PVDF-bound known protein with a homogeneous amino-terminus.

The Association of Biomolecular Resource Facilities 2003 Edman Sequencing Research Group (ABRF-ESRG'03) sample is the 15th in a series of studies designed to allow participating members to evaluate their abilities to analyze the N-terminus of a protein or peptide using automated Edman degradation chemistry. It is a follow-up study to the ESRG'02 sample, which was a single protein with a heterogeneous N-terminus. Both the 2002 and 2003 samples were obtained from the same protein complex and were resolved by SDS-PAGE followed by electrophoretic transfer to PVDF membrane.

Design of PEGylated soluble tumor necrosis factor receptor type I (PEG sTNF-RI) for chronic inflammatory diseases.

A recombinant C-terminal truncated form of the human soluble tumor necrosis factor receptor type I (sTNF-RI) was produced in E. coli. This soluble receptor contains the first 2.