Genetically encoded protein oscillators for FM streaming of single-cell data.

Radios and cellphones use frequency modulation (FM) of an oscillating carrier signal to reliably transmit multiplexed data while rejecting noise. Here, we establish a biochemical analogue of this paradigm using genetically encoded protein oscillators (GEOs) as carrier signals in circuits that enable continuous, real-time FM streaming of single-cell data. GEOs are constructed from evolutionarily diverse MinDE-family ATPase and activator modules that generate fast synthetic protein oscillations when co-expressed in human cells.

Urinary metabolite model to predict the dying process in lung cancer patients.

Background: Accurately recognizing that a person may be dying is central to improving their experience of care at the end-of-life. However, predicting dying is frequently inaccurate and often occurs only hours or a few days before death.

Methods: We performed urinary metabolomics analysis on patients with lung cancer to create a metabolite model to predict dying over the last 30 days of life.

Synthetic forms most beautiful: engineering insights into self-organization.

Reflecting on the diversity of the natural world, Darwin famously observed that "from so simple a beginning endless forms most beautiful and most wonderful have been, and are being evolved". However, the examples that we are able to observe in nature are a consequence of chance, constrained by selection, drift and epistasis. Here we explore how the efforts of synthetic biology to build new living systems can expand our understanding of the fundamental design principles that allow life to self-organize biological form, from cellular to organismal levels.

A Review of Long-Term Outcomes of Liver Transplantation Using Extended Criteria Donors in the United States.

Introduction: Utilizing the marginal livers for transplantation has gained more attention recently, but there are still some doubts regarding the outcomes. This study focuses on the outcomes of extended criteria donor liver transplants (LTs) in the United States, in order to assess the feasibility of these types of livers utilization.

Materials And Methods: A retrospective registry analysis of the Organ Procurement and Transplantation Network/United Network for Organ Sharing database was done for LTs that were performed in the United States from January 2001 to April 2020.

Dysregulated Clot Mechanics and Kinetics Impacted by Injury Severity, Predict Mortality After Trauma.

Introduction: Coagulopathy following traumatic injury impairs stable blood clot formation and exacerbates mortality from hemorrhage. Understanding how these alterations impact blood clot stability is critical to improving resuscitation. Furthermore, the incorporation of machine learning algorithms to assess clinical markers, coagulation assays and biochemical assays allows us to define the contributions of these factors to mortality.