The measurement of AgNORs in human glioma cells.

Sixty gliomas obtained by neurosurgical resections were examined. Paraffin blocks were retrieved from pathological files of the Second Affiliated Hospital in Guangzhou Medical College. The methods of argyrophilic technique for AgNORs staining, and Image Analysis System for measurement of AgNORs were used.

Bcl-2 and Bax proteins are nuclear matrix associated proteins.

Bcl-2 and Bax proteins are implicated in the regulation of apoptosis. Nuclear matrix has been demonstrated to be associated with a vast array of functional and regulatory properties of cells. NuMA is one member of a class of nuclear matrix proteins that resides in both the nucleus and mitotic apparatus.

Expression of bcl-2 and Bax in EGFR-antisense transfected and untransfected glioblastoma cells.

Glioblastomas are the most frequent and most malignant astrocytic gliomas. The epidermal growth factor receptor (EGFR) gene is the most frequently amplified and overexpressed in glioblastomas. The expression of bcl-2 and Bax in EGFR-antisense transfected and un-transfected glioblastoma cell line, U87E and U87V was studied by immunohistochemistry and western blotting.

Effects of all-trans-retinoic acid on human esophageal carcinoma cells and their nuclear matrices.

Esophageal cancer is a common cancer among ethnic Chinese. The reported incidence of esophageal cancer has increased many fold in the past decades and is apparently still rising. Nuclear matrix forms the scaffold of the nucleus and participates in various nuclear functions.

Association of EGFR gene fragments with nuclear matrices in glioblastoma cell lines.

The association of epidermal growth factor receptor DNA fragments with nuclear matrix in glioblastoma cell lines was investigated. Two different DNA fragments (primer I-III, 940 bp and primer IV-V, 110 bp) were amplified in both genomic DNA and nuclear matrix-associated DNA. It was found that the 110 bp DNA fragment (primer IV-V) from a non-encoding region was more closely associated with the nuclear matrices of cell line U343, U373, A172, and T98 than with U87.