Antigen receptor ITAMs provide tonic signaling by acting as guanine nucleotide exchange factors to directly activate R-RAS2.

The small GTPase R-RAS2 regulates homeostatic proliferation and survival of T and B lymphocytes and, when present in high amounts, drives the development of B cell chronic lymphocytic leukemia. In normal and leukemic lymphocytes, R-RAS2 constitutively binds to antigen receptors through their immunoreceptor tyrosine-based activation motifs (ITAMs) and promotes tonic activation of the phosphatidylinositol 3-kinase (PI3K) signaling pathway. Here, we examined the molecular mechanisms underlying this direct interaction and its consequences for R-RAS2 activity.

Improving photosynthetic biogas purification via process aeration and nanoparticle supplementation.

This study evaluated the influence of nanoparticles in both suspension and solid format on the performance of a microalgal process devoted to photosynthetic biogas purification. The experimental system consisted of an enclosed tubular photobioreactor coupled to a biogas absorption column through a mixing chamber. The high NH concentration in the inlet mineral medium (530 mg N-NH L) and the punctual addition of 115 mL of nanoparticle suspension to the system caused inhibition of the microalgal-bacterial cultivation.

Native RNA nanopore sequencing reveals antibiotic-induced loss of rRNA modifications in the A- and P-sites.

The biological relevance and dynamics of mRNA modifications have been extensively studied; however, whether rRNA modifications are dynamically regulated, and under which conditions, remains unclear. Here, we systematically characterize bacterial rRNA modifications upon exposure to diverse antibiotics using native RNA nanopore sequencing. To identify significant rRNA modification changes, we develop NanoConsensus, a novel pipeline that is robust across RNA modification types, stoichiometries and coverage, with very low false positive rates, outperforming all individual algorithms tested.

Silent St. Louis encephalitis virus circulation evidence by a haemovigilance survey in a centralized blood bank.

Background: Saint Louis encephalitis virus (SLEV) re-emergence and its geographical expansion, evidenced by its emergence in previously unaffected areas, have raised significant public health concerns. We aimed to show the usefulness of haemovigilance as an effective tool to fill arboviruses surveillance gaps to track trends and identify hotspots.

Methods: Within the framework of a blood bank haemovigilance program, we performed a survey to evaluate the potential threat of circulating mosquito-borne infections for transfusion safety.