The structure of the O-specific polysaccharide from Thiobacillus sp. IFO 14570, with three different diaminopyranoses forming the repeating unit.

The O-specific polysaccharide, liberated by mild acid hydrolysis of the lipopolysaccharide (LPS-P) from Thiobacillus sp. IFO14570 as isolated from the phenol phase after phenol-water extraction, is shown to have a linear trisaccharide repeating-unit containing three different diamino sugars, namely 2,4-diacetamido-2,4-dideoxyglucuronic acid, 2-acetamidino-4-acetamido-2,4,6-trideoxyglucopyranose, and 2,3-diacetamido-2,3-dideoxyglucuronic acid in the molar ratio of 1:1:1. On the basis of 1H and 13C spectroscopy, including 2D COSY and coherent transfer (RCT)COSY, 1D NOE in the difference mode, and 2D rotating frame NOE(ROESY) the sequence, the type of substitution, and the position of the acetamidino group could be determined.

The structure of the O-specific polysaccharide from Thiobacillus ferrooxidans IFO 14262.

Lipopolysaccharide (LPS) was isolated from Thiobacillus ferrooxidans IFO 14262 by the hot phenol-water extraction procedure. The O-specific polysaccharide, liberated from LPS by mild acetic acid hydrolysis, had a branched pentasaccharide repeating-unit composed of D-glucose, L-rhamnose, D-rhamnose, and 3-O-methyl-L-rhamnose in approximate molar ratios of 2:1:1:1. On the basis of methylation analysis, 1H and 13C NMR spectroscopy, including 2D shift-correlated (COSY) and 1D NOE spectroscopy, the structure for the repeating unit of the O-specific polysaccharide was established, and the assumed biological repeating unit indicated.

Parallel induction of nitric oxide and glucose-6-phosphate dehydrogenase in activated bone marrow derived macrophages.

The production of nitric oxide (NO.) and the induction of glucose-6-phosphate dehydrogenase by lipopolysaccharides (LPS) from different sources was studied in bone marrow derived macrophages (BMM phi). NO.