Roadmap and Considerations for Genome Editing in a Non-Model Organism: Genetic Variations and Off-Target Profiling.

The CRISPR/Cas genome editing approach in non-model organisms poses challenges that remain to be resolved. Here, we demonstrated a generalized roadmap for a de novo genome annotation approach applied to the non-model organism . We also addressed the typical genome editing challenges arising from genetic variations, such as a high frequency of single nucleotide polymorphisms, differences in sex chromosomes, and repetitive sequences that can lead to off-target events.

Reichsanzeiger-GT: An OCR ground truth dataset based on the historical newspaper "Deutscher Reichsanzeiger und Preußischer Staatsanzeiger" (German Imperial Gazette and Prussian Official Gazette) (1819-1945).

Reichsanzeiger-GT is a ground truth dataset for OCR training and evaluation based on the historical German newspaper "Deutscher Reichsanzeiger und Preußischer Staatsanzeiger" (German Imperial Gazette and Prussian Official Gazette), which was published from 1819 to 1945 and printed mostly in the typeface Fraktur (Black Letter). The dataset consists of 101 newspaper pages for the years 1820-1939, that cover a wide variety of topics, page layouts (lists, tables, and advertisements) as well as different typefaces. Using the transcription software Transkribus and the open-source OCR engine Tesseract we automatically created and manually corrected layout segmentations and transcriptions for each page, resulting in 65,563 text regions, 412 table regions, 119,429 text lines and 490,679 words.

A transporter that allows phosphate ions to control the polymorph of exoskeletal calcium carbonate biomineralization.

The SLC20A2 transporter supplies phosphate ions (P) for diverse biological functions in vertebrates, yet has not been studied in crustaceans. Unlike vertebrates, whose skeletons are mineralized mainly by calcium phosphate, only minute amounts of P are found in the CaCO-mineralized exoskeletons of invertebrates. In this study, a crustacean SLC20A2 transporter was discovered and P transport to exoskeletal elements was studied with respect to the role of P in invertebrate exoskeleton biomineralization, revealing an evolutionarily conserved mechanism for P transport in both vertebrates and invertebrates.

Functional studies with IgM and IgA immunoglobulins: binding to pIgR, FcαμR, FcμR, and CDC activities.

IgMs are the first antibodies produced by the immune system upon encounter of a possible pathogen and are one of five antibody subclasses in humans. For IgG, the most intensively studied antibody class, the N-linked glycosylation site located in the Fc-domain is directly involved in high affinity binding to the respective receptors and initiation of corresponding immune response. IgM molecules have five N-glycosylation sites and one N-glycosylation site in the J-chain, which can be incorporated in IgM or IgA molecules.