Respiratory inhibitors and uncouplers prevent the aeration-induced increase in mitochondrial anion conductivity.

1. When mitochondria are stirred in air the rate of anion conductivity increases, this effect being enhanced by the addition of respiratory substrate. 2.

Palmitoyl-CoA inhibits the mitochondrial inner membrane anion-conducting channel.

Palmitoyl-CoA is shown here to inhibit the pH-dependent anion-conducting channel (IMAC) in the inner membrane of rat liver mitochondria, with half-maximal inhibition at 2.4 microM. It has little effect on the transport of ribose, thiocyanate and glutamate.