Coenzyme Q10 does not enhance preadipocyte viability in an in vitro lipotransfer model.

Background: Autologous fat is an attractive soft-tissue filler in plastic and reconstructive surgery. The success of the procedure relies strongly on the technique of transferring viable preadipocytes. Among other factors, preadipocyte viability is impaired by local anesthetics.

Local anesthetics have a major impact on viability of preadipocytes and their differentiation into adipocytes.

Background: Autologous fat transplantation is a well-established technique in surgery. Moreover, the use of preadipocytes in soft-tissue engineering is currently being intensely investigated. Current efforts focus on identifying maneuvers that may minimize resorption and provide predictable late results.

Construction of a multi-layer skin substitute: Simultaneous cultivation of keratinocytes and preadipocytes on a dermal template.

Background: After deep excision of burn eschar down to the muscle fascia patients have a non-reversible loss of the skin and underlying subcutaneous tissue. These patients would benefit from the development of a sufficient epidermal, dermal, and hypodermal tissue-engineered replacement provided by new technologies of tissue engineering. The aim of the present study was to determine whether keratinocytes and preadipocytes grow simultaneously on a bovine-derived collagen-elastin matrix under in vitro conditions in order to obtain a multi-layer skin substitute.

[In-vitro suspension model of haemangioma vascular endothelial cells (HVECs)].

Introduction: Haemangioma is the most common tumour of infancy. Its biological behaviour is one of a benign nature and characterised by a typical biphasic growth pattern. After an initial proliferation during the child's first year of life an involution takes place and usually lasts until the first decade.

Cultured human epithelium: human umbilical cord blood stem cells differentiate into keratinocytes under in vitro conditions.

Background: Stem cells have the capacity to renew or to give rise to a specialized cell types. Human umbilical cord blood (HUCB) has been explored as an alternative source of stem cells. However, its potential to differentiate into cells of other tissues is still under discussion.