An HPA-1a-positive platelet-depleting agent for prevention of fetal and neonatal alloimmune thrombocytopenia: a randomized, single-blind, placebo-controlled, single-center, phase 1/2 proof-of-concept study.

Background: Fetal/neonatal alloimmune thrombocytopenia (FNAIT) is a rare and potentially life-threatening bleeding disorder of the fetus/newborn. Antibodies against human platelet antigen 1a (HPA-1a) are associated with the most frequent FNAIT cases. There are no approved therapies for FNAIT prevention or treatment.

Focal cerebral ischemia.

Rodent models of focal cerebral ischemia have been extremely useful in elucidating pathomechanisms of human stroke. Most commonly, a monofilament is advanced through the internal carotid artery of rodents to occlude the origin of the middle cerebral artery thus leading to critical ischemia in the corresponding vascular territory. The filament can be removed after different occlusion times allowing reperfusion (transient middle cerebral artery occlusion (MCAO) model) or is left permanently within the internal carotid artery (permanent MCAO model) both mimicking clinical thromboembolic stroke in which the occluding clot may resolve spontaneously or after thrombolysis, or may persist.

α(2)-adrenoceptors do not mediate neuroprotection in acute ischemic stroke in mice.

We assessed the neuroprotective potential of α(2)-adrenoceptors in ischemic stroke using mice with targeted deletions of individual α(2)-adrenoceptor subtypes (α(2A)(-/-), α(2B)(-/-), α(2C)(-/-), α(2A/C)(-/-)). The effects of the α(2)-adrenoceptor agonist clonidine were studied in parallel. Focal cerebral ischemia was induced with or without clonidine pretreatment by transient middle cerebral artery occlusion.

Outcome of experimental stroke in C57Bl/6 and Sv/129 mice assessed by multimodal ultra-high field MRI.

Transgenic mice bred on C57Bl/6 or Sv/129 genetic background are frequently used in stroke research. It is well established that variations in cerebrovascular anatomy and hemodynamics can influence stroke outcome in different inbred mouse lines. We compared stroke development in C57Bl/6 and Sv/129 mice in the widely used model of transient middle cerebral artery occlusion (tMCAO) by multimodal ultra-high field magnetic resonance imaging (MRI).