Proteomic Analysis of Rap1A GTPase Signaling-Deficient C57BL/6 Mouse Pancreas and Functional Studies Identify an Essential Role of Rap1A in Pancreas Physiology.

Ras-related Rap1A GTPase is implicated in pancreas β-cell insulin secretion and is stimulated by the cAMP sensor Epac2, a guanine exchange factor and activator of Rap1 GTPase. In this study, we examined the differential proteomic profiles of pancreata from C57BL/6 Rap1A-deficient (Null) and control wild-type (WT) mice with nanoLC-ESI-MS/MS to assess targets of Rap1A potentially involved in insulin regulation. We identified 77 overlapping identifier proteins in both groups, with 8 distinct identifier proteins in Null versus 56 distinct identifier proteins in WT mice pancreata.

Identifying Medical Students in Difficulty Using Integration of Programmatic Assessment and Real-Time Evaluation Framework.

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Optimising Preeclampsia First-Trimester Screening Using Three Parameters.

Objective: To evaluate the performance of first-trimester preeclampsia-screening algorithm in predicting preeclampsia (PE).

Study Design: Observational study. Place and Duration of the Study: Department of Obstetrics and Gynaecology, Combined Military Hospitals (CMH) Lahore, Pakistan, between 1st January and 31st August 2022.

A Multi-centre Experience of Trans-abdominal Chorionic Villus Sampling in Pakistan.

Objective: To determine the safety and outcomes of trans-abdominal chorionic villus sampling technique.

Study Design: Observational study.

Place And Duration Of Study: Departments of Obstetrics and Gynaecology, PNS Shifa Karachi, Pak-Emirates Military Hospital Rawalpindi and CMH Lahore, from 2005-2020.