Health Care Personnel Attitudes Toward Pediatric COVID-19 Vaccines.

Introduction: A level of hesitancy existed among parents when United States Food and Drug Administration (FDA)-approved pediatric COVID-19 vaccines were introduced. We explored attitudes, beliefs, and willingness of health care personnel (HCP) as parents to vaccinate children less than 18 years of age.

Methods: We developed a cross-sectional survey for HCPs as parents, including clinical and nonclinical staff, researchers, and trainees at a single academic medical institution.

Effects of volume management on free flap perfusion and metabolism in a large animal model study.

Free flap failure represents a substantial clinical burden. The role of intraoperative volume management remains controversial, with valid studies lacking. Here, using a large animal model, we investigated the influence of volume management on free flap perfusion and metabolism.

Long-Lived Coherence on a μHz Scale Optical Magnetic Quadrupole Transition.

We report on the coherent excitation of the ultranarrow ^{1}S_{0}-^{3}P_{2} magnetic quadrupole transition in ^{88}Sr. By confining atoms in a state insensitive optical lattice, we achieve excitation fractions of 97(1)% and observe linewidths as narrow as 58(1) Hz. With Ramsey spectroscopy, we find coherence times of 14(1) ms, which can be extended to 266(36) ms using a spin-echo sequence.

Single-cell nascent RNA sequencing unveils coordinated global transcription.

Transcription is the primary regulatory step in gene expression. Divergent transcription initiation from promoters and enhancers produces stable RNAs from genes and unstable RNAs from enhancers. Nascent RNA capture and sequencing assays simultaneously measure gene and enhancer activity in cell populations.