Enhancement of growth and ferrous iron oxidation rates of T. Ferrooxidans by electrochemical reduction of ferric iron.

Thiobacillus ferrooxidans, the bacterium most widely used; in bioleaching or microbial desulfurization studies, was grown in an electrolytic bioreactor containing a synthetic, ferrous sulfate medium. Passage of current through the medium reduced the bacterially generated ferric iron to the ferrous iron substrate. When used in conjunction with an inoculum that had been adapted to the electrolytic growth conditions, this technique increased the protein (cell) concentration by 3.

Mg2+- or Mn2+-dependent p-nitrophenylphosphatase activity is present in Ehrlich ascites tumor cells.

The presence of a soluble, Mg2+- or Mn2+-dependent p-nitrophenylphosphatase activity in Ehrlich ascites tumor cell homogenates is reported. The crude homogenate was fractionated over Sephadex G-150 gel-filtration and DEAE-Sephacel anion-exchange columns, and two p-nitrophenylphosphatase activities were resolved. The most active fraction, Peak I, was characterized and found to be similar to phosphotyrosyl-protein phosphatases characterized elsewhere in that it has optimal activity at neutral pH; it is inhibited by phosphate, Zn2+, and vanadate; and it is not inhibited by levamisole.