The first nucleotide binding domain of the sulfonylurea receptor 2A contains regulatory elements and is folded and functions as an independent module.

The sulfonylurea receptor 2A (SUR2A) is an ATP-binding cassette (ABC) protein that forms the regulatory subunit of ATP-sensitive potassium (K(ATP)) channels in the heart. ATP binding and hydrolysis at the SUR2A nucleotide binding domains (NBDs) control gating of K(ATP) channels, and mutations in the NBDs that affect ATP hydrolysis and cellular trafficking cause cardiovascular disorders. To date, there is limited information on the SUR2A NBDs and the effects of disease-causing mutations on their structure and interactions.

Biomimetic aminoacylation of ribonucleotides and RNA with aminoacyl phosphate esters and lanthanum salts.

Aminoacylation of tRNA in cells involves activation of the amino acid as an aminoacyl adenylate, a mixed anhydride with AMP, which reacts with tRNA. We have now established that aminoacyl phosphate esters in the presence of lanthanide ions in water will acylate hydroxyls at the 3'-terminus of RNA or a simple nucleotide. By extension, this will permit synthetically aminoacylated tRNA to be produced in a single-step biomimetic process.

Influence of age, sex and body weight on renal osteodystrophy in predialysis patients with chronic renal failure.

Unlabelled: The aim of the present investigation was to examine the influence of age, sex and body weight on osseous changes in pre-dialysis patients with chronic renal failure (CRF). 87 patients (44 males and 43 females) aged 18-60 years with CRF were studied. The levels of serum creatinine, total and ionized calcium, phosphorus, alkaline phosphatase, intact parathormone and serum osteocalcine were followed up.