Publications by authors named "S B Panina"
The C-terminal domain of RPB1 (CTD) orchestrates transcription by recruiting regulators to RNA Pol II upon phosphorylation. With CTD driving condensate formation on gene loci, the molecular mechanism behind how CTD-mediated recruitment of transcriptional regulators influences condensates formation remains unclear. Our study unveils that phosphorylation reversibly dissolves phase separation induced by the unphosphorylated CTD.
View Article and Find Full Text PDFRNA polymerase II relies on a repetitive sequence domain (YSPTSPS) within its largest subunit to orchestrate transcription. While phosphorylation on serine-2/serine-5 of the carboxyl-terminal heptad repeats is well established, threonine-4's role remains enigmatic. Paradoxically, threonine-4 phosphorylation was only detected after transcription end sites despite functionally implicated in pausing, elongation, termination, and messenger RNA processing.
View Article and Find Full Text PDFBackground: Glioblastoma (GBM) is an aggressive brain cancer associated with poor prognosis, intrinsic heterogeneity, plasticity, and therapy resistance. In some GBMs, cell proliferation is fueled by a transcriptional regulator, repressor element-1 silencing transcription factor (REST).
Results: Using CRISPR/Cas9, we identified GBM cell lines dependent on REST activity.
The C-terminal domain of RPB1 (CTD) orchestrates transcription by recruiting regulators to RNA Pol II upon phosphorylation. Recent insights highlight the pivotal role of CTD in driving condensate formation on gene loci. Yet, the molecular mechanism behind how CTD-mediated recruitment of transcriptional regulators influences condensates formation remains unclear.
View Article and Find Full Text PDFArticle Synopsis
- RNA polymerase II (Pol II) undergoes important post-translational modifications on its C-terminal domain (CTD), which help regulate transcription by attracting different proteins at various stages of the transcription process.
- *The phosphorylation of specific serine residues (Ser5 and Ser2) on the CTD is crucial, as it occurs in relation to the transcription phases and influences which transcriptional regulators bind to Pol II.
- *The study identified calcium homeostasis endoplasmic reticulum protein (CHERP) as a significant regulatory protein that associates with the phosphorylated CTD, impacting alternative splicing when disrupted.