Image analysis of bioparticles accumulation and diamagnetic alignment in high-gradient magnetic field.

Magnetic properties of biological particles are measured in high-gradient magnetic separation (HGMS) analysis, revealing the concentrating process of nucleoprotein particles, ferritin, red blood cells, and eggs. A magnetic force acting on micrometer and submicrometer biological particles having diamagnetic or paramagnetic susceptibility with respect to the solution causes their movement and accumulation in gradient magnetic fields dependent on the values of the magnetic moments. The methods developed enable us to obtain the magnetic moments values of single particles and their assembly directly from magnetic separation and image analyses without assuming the detection of sizes.

[Concentration-dependent effects of magnetic accumulation of ligand-binding microparticles and ferritin aggregates].

A method for measuring the magnetic susceptibility of microparticles of a suspension from the thickness of the layer deposited on the surface of a magnetized wire was developed. The magnetic accumulation was registered by means of a photodetector, by measuring the amplitude and spectrum of fluctuations of the intensity of scattered light. This was used as the basis for the development of a biosensor and biochips.

[Magnetic susceptibility and magnetic capture of cells].

A phenomenon of magnetic capture of human erythrocytes and lymphocytes in paramagnetic and diamagnetic modes of motion near a transversely magnetized wire has been investigated. The values of magnetic susceptibility of numerous individual erythrocytes containing hemoglobin in various forms and lymphocytes were determined using a trajectory analysis. The distribution histograms of erythrocytes in various states and lymphocytes of magnetic susceptibility have been constructed.

[Magnetic susceptibility of single human erythrocytes].

Velocity of movement of a large number of erythrocytes containing different forms of hemoglobin in a homogeneous magnetic field was measured. Distributions of the erythrocytes according to their movement velocity were obtained. Values of magnetic susceptibility were found.

[Interaction of the Embden-Meyerhof pathway and hexose monophosphate shunt in erythrocytes].

A mathematical model of glycolysis in human erythrocytes for the interaction between the Embden-Meyerhof and the pentose phosphate pathways has been developed. The characteristic surfaces, i. e.