Apical periodontitis microbiome association with salivary and serum inflammatory burden.

Aims: Apical Periodontitis (AP) involves complex interactions between the root canal microbiome and the host immune response, with potential risk of local and systemic inflammatory burden, however there is no evidence available regarding correlation between microbiome and inflammatory marker levels. This study aims to identify the microbiome of saliva, intracanal and blood samples in AP subjects and investigate the correlation between intracanal and blood microbiomes with serum inflammatory biomarker levels, and salivary microbiomes with salivary inflammatory biomarker levels.

Methodology: Saliva, Intracanal and blood samples were collected from AP patients undergoing root canal retreatment.

MicroRNA Nobel Prize: Timely Recognition and High Anticipation of Future Products-A Prospective Analysis.

MicroRNAs (miRNAs) maintain cellular homeostasis by blocking mRNAs by binding with them to fine-tune the expression of genes across numerous biological pathways. The 2024 Nobel Prize in Medicine and Physiology for discovering miRNAs was long overdue. We anticipate a deluge of research work involving miRNAs to repeat the history of prizes awarded for research on other RNAs.

Advancing Therapeutic and Vaccine Proteins: Switching from Recombinant to Ribosomal Delivery-A Humanitarian Cause.

Recombinant therapeutic and vaccine proteins have revolutionized healthcare, but there remain challenges, as many are awaiting development due to their slow development speed and high development cost. Cell-free in vivo ribosomes offer one choice, but they come with similar constraints. The validation of in vivo messenger RNA (mRNA) technology has been accomplished for COVID-19 vaccines.

Detection of Rotavirus, Norovirus, and Astrovirus Causing Acute Diarrhoea in Children by Multiplex PCR in a Tertiary Care Hospital.

Objective: To evaluate the use of a multiplex real-time polymerase chain reaction (RT-PCR) test in detecting three viruses namely Rotavirus, Norovirus (genotypes 1 and 2), and Astrovirus that cause gastroenteritis in children under the age of five years.

Study Design: A cross-sectional study. Place and Duration of the Study: Department of Virology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, Pakistan, from January to July 2023.

Doped magnetic nanoparticles: From synthesis to applied technological frontiers.

Doped magnetic nanoparticles (DMNPs) have become a fascinating class of nanomaterials with important implications in science and technology. The comprehensive review focuses on the synthetic methods, types of doping elements, distinctive properties, and extensive applications of DMNPs. The synthesis section highlights different methods, highlighting their benefits and drawbacks, such as chemical precipitation, co-precipitation, thermal breakdown, sol-gel, and other processes.