[The development of a rapid semiautomatic enzymatic analyzer of beta-lactam antibiotics].

A new method and a device for enzymatic express assay of beta-lactam antibiotics are described. The principle of the device operation is based on the use of native enzymes specific to the antibiotics assayed. The method makes it possible to exclude the influence of the buffer capacity of the samples on the results of the assay.

[A potentiometric rapid method of determining the level of phenylacetic acid during the production of beta-lactam antibiotics].

A potentiometric rapid method for control of phenylacetic acid (PAA) concentration in production of ++beta-lactam antibiotics is described. The method is based on ion selective electrodes with a film membrane. The results of the theoretical and experimental studies on estimation of the electrode selectivity specific of PAA in the presence of various interfering ions are presented.

[Elaboration of a semiautomated enzymatic analyzer of glucose for the control of biosynthesis of antibiotics].

The results of developing a semiautomatic apparatus with oxygen detection for enzymatic control of glucose concentration are presented. The design of a glucose sensitive electrode is based on an oxygen probe and a membrane with immobilized glucose oxidase. Materials for the probe were chosen and the operating conditions for measuring temperature, pH, linear agitation velocity and other parameters were optimized.

[Absorption spectrum of admixtures coloring butyl acetate extract].

Colour of butyl acetate extract (BAE) at the clarification stage in chemical purification of benzylpenicillin is one of the important qualitative parameters necessary for the process control. BAE is benzylpenicillin-enriched butyl acetate with colouring pigments and admixtures of unknown nature. The routine laboratory photocolorimetric method provides only periodical control.

[Penicillin-selective electrode based on a cellulose membrane with immobilized enzyme].

Modified cellulose films are recommended to be used as carriers in preparing enzymatic membranes for penicillin-selective electrodes. The results of laboratory testing of the enzymatic penicillin-selective electrode based on the cellulose membrane with immobilized penicillinase are presented. In principle it was shown possible to use the enzymatic electrode in determining penicillin concentration in solutions.