α-ketoglutarate ameliorates colitis through modulation of inflammation, ER stress, and apoptosis.

Colitis is an inflammatory disorder of the gastrointestinal tract. A widely consumed dietary nutrient, α-ketoglutarate (α-KG) is known to play a crucial role in cellular metabolism and provide protection to intestinal epithelium under various pathophysiological conditions. In this study, 2,4,6-trinitrobenzenesulfonic acid (TNBS) was used to induce colitis in Wistar rats.

A physicochemical investigation of the complex formation by β-cyclodextrin with Triton X-100 and Triton X-114 and their aggregation behaviour in aqueous solution: an experimental approach.

The complexation behavior and binding affinity of Triton X-100 (TX-100) and Triton X-114 (TX-114) with β-cyclodextrin (β-CD) were extensively studied in an aqueous medium using a comprehensive suite of experimental techniques. These techniques allowed for the evaluation of key physicochemical parameters, including critical micelle concentration (cmc), aggregation number (), Stern-Volmer constant, and particle size distribution. These metrics were instrumental in understanding the underlying mechanism of the host-guest interaction between β-CD and Triton-X.

Oncolytic reprogramming of tumor microenvironment shapes CD4 T-cell memory via the IL6ra-Bcl6 axis for targeted control of glioblastoma.

Oncolytic viruses (OVs) emerge as a promising cancer immunotherapy. However, the temporal impact on tumor cells and the tumor microenvironment, and the nature of anti-tumor immunity post-therapy remain largely unclear. Here we report that CD4 T cells are required for durable tumor control in syngeneic murine models of glioblastoma multiforme after treatment with an oncolytic herpes simplex virus (oHSV) engineered to express IL-12.

Multiplexing Label-Free Polymeric Nanocarriers via Antipolymer Antibodies.

Recent examples of immune responses directed against the synthetic polymer poly(ethylene glycol) (PEG) have led to the development of biocompatible polymers, which are viewed as promising candidates to act as surrogate materials for use in biological applications, such as hydrophilic poly(2-oxazoline)s (POx). Despite this, the characterization of critical aspects of the immune response against these emerging materials is sparse, in part because no known monoclonal antibodies (mAbs) against this family of synthetic material have been reported. To advance the understanding of such responses, we report the successful isolation and characterization of hybridoma-derived mAbs with excellent specificity for different POx species and notable selectivity for highly branched polymer architectures over linear systems.

Characterization and anti-biofilm potentiality of an isolated novel Aeromonas hydrophila-infecting bacteriophage AHPMCC11, belonging to the genus Ahphunavirus.

Aeromonas hydrophila is a major aquatic habitat pathogen responsible for huge economic losses in the aquaculture and food industries. In this study, a lytic bacteriophage AHPMCC11 was isolated by using A. hydrophila MTCC 1739.