Heterologous expression, purification, and biochemical characterization of protease 3075 from Cohnella sp. A01.

Proteases as one of the most significant categories of commercial enzymes, serve nowadays as the key ingredients in detergent formulations. Therefore, identifying detergent-compatible proteases with better properties is a continuous exercise. Accordingly, we were interested in the recombinant production and characterization of protease 3075 as a novel enzyme from thermophilic indigenous Cohnella sp.

Emergence of lineage ST150 and linezolid resistance in : a molecular epidemiology study of UTIs in Tehran, Iran.

Background: Urinary tract infections (UTIs) represent one of the most prevalent bacterial infections, with Enterococcus species now recognized as the second leading cause of these infections. This study focused on symptomatic UTI cases to investigate the risk factors associated with clinical isolates in patients from Tehran, Iran.

Methods: Urine samples were collected from patients presenting with symptomatic UTIs.

A novel thermophilic lysozyme 4356 from Cohnella sp. A01: Cloning, heterologous expression, biochemical and kinetic characterization.

Lysozymes have gained attention for their antiseptic properties. In silico studies have shown that the enzyme containing lysM can act as an antibacterial agent. Binding of the lysM motif of rSELys to peptidoglycan and molecular dynamics simulations showed that the protein-ligand binding is very stable.

Expression optimization and characterization of a novel amylopullulanase from the thermophilic Cohnella sp. A01.

Amylopullulanase (EC. 3.2.

Protective Effects of Zingerone on Oxidative Stress in Doxorubicin-Induced Rat Hepatotoxicity.

Background: Doxorubicin, a commonly utilized anthracycline antibiotic and chemotherapeutic agent, has been associated with hepatotoxicity as an adverse effect. This study aimed to evaluate protective effects of zingerone, a bioactive compound derived from ginger renowned for its antioxidative attributes, on oxidative stress in doxorubicin-induced rat hepatotoxicity.

Methods: In this experimental study, a total of 48 male Wistar rats were allocated into six distinct groups.