[Preparation and immunochemical properties of monoclonal antibodies to potato ring rot pathogen Corynebacterium sepedonicum].

Five stable hybridoma lines producing monoclonal antibodies to Corynebacterium sepedonicum were obtained. The specificity of monoclonal antibodies obtained was characterized. Interactions of the antibodies with native cells and antigenic preparations from bacterial cell extracts were studied.

Highly sensitive immunoassays for detection of barley stripe mosaic virus and beet necrotic yellow vein virus.

Enzyme immunoassays based on the use of monoclonal antibodies (MAbs) were developed for the detection of the barley stripe mosaic virus (BSMV) and the beet necrotic yellow vein virus (BNYVV). Assays employing conjugates of MAbs to horseradish peroxidase (HRP) were compared to systems with biotinylated MAbs and streptavidin conjugated either to monomeric HRP or to HRP homopolymers with different polymerisation degrees including those of 20, 40 and 80. In the ELISA with streptavidin-polymeric HRP conjugates the assay detection limit was about 12-25 times as good as in the monoclonal double antibody sandwich ELISA.

[A comparative analysis of the expression of the betaEst2 gene in the ontogeny of the Drosophila virilis group].

We studied expression of gene betaEst2 controlling synthesis of non-specific carboxylesterase (EC 3.1.1.