Histologic and immunocytochemical characterization of canine distemper-associated metaphyseal bone lesions in young dogs following experimental infection.

The proximal metaphyses of the humerus of weanling gnotobiotic dogs experimentally infected with canine distemper virus (CDV) were investigated histologically and immunocytochemically between 4 and 41 days after infection. Viral antigen was demonstrated in hematopoietic marrow and bone cells at postinfection day (PID) 5 and PID 7, respectively. Between PID 8 and 27, CDV antigen was abundantly present in marrow cells, osteoclasts, and osteoblasts and less frequently in osteocytes.

Characterization of a novel inositol 1,4,5-trisphosphate receptor in isolated olfactory cilia.

Inositol 1,4,5-trisphosphate (InsP3), a product of G-protein-mediated receptor activation of phosphoinositide turnover, plays the role of a second messenger when olfactory neurons are stimulated with certain olfactory stimuli. In this paper we examine the specific binding of [3H]InsP3 to isolated olfactory cilia, microsomes and brain membranes from the channel catfish (Ictalurus punctatus) and, by photoaffinity labelling with an InsP3 analogue (125I-labelled 1-[3-(4-azidosalicyloxy)-aminopropyl]inositol 1,4,5-trisphosphate (125I-ASA-InsP3)], we tentatively identify the major InsP3-binding protein in catfish olfactory cilia. InsP3 binding to ciliary membranes is specific and saturable, with a Kd of 1.

Analysis of lung air from patients with bronchogenic carcinoma and controls using gas chromatography-mass spectrometry.

Volatile metabolites present in expired lung air were collected by odor sampling techniques and analyzed by gas chromatography-mass spectrometry. The study population included controls matched for age and smoking history with patients newly diagnosed with lung carcinoma. Significantly greater concentrations of o-toluidine were found in the lung air of patients with lung carcinoma than either age-matched or younger controls.

Characterization of pathogenic bacteria by automated headspace concentration-gas chromatography.

Automated headspace concentration-gas chromatography (AHC-GC) was used to profile the volatile metabolites produced by Proteus mirabilis, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. Bacterial cultures were incubated in trypticase soy broth and examined at 24 h. The profiles were consistent for each genus examined and variation observed among the different strains of each species was chiefly quantitative.