Background: Serum progesterone (P) rises after ovulation in the luteinisation process.
Objective: To identify an accurate progesterone threshold to confirm ovulation in the assessment of a woman's fertility.
Methods: In a secondary analysis of an observational European multicentre study, this study included 107 women over 326 menstrual cycles and tracked daily first morning urine (FMU), changes in observed cervical mucus discharge, serum progesterone, and ultrasonography to identify the day of ovulation.