Reproducibility problems with the AMPLICOR PCR Chlamydia trachomatis test.

In an attempt to use an expanded "gold standard" in an evaluation of an antigen detection test for Chlamydia trachomatis, the AMPLICOR (Roche Diagnostics Systems, Inc., Branchburg, N.J.

Comparison of the ProSpecT and Color Vue enzyme-linked immunoassays for the detection of Cryptosporidium in stool specimens.

Two enzyme-linked immunosorbent assays, the ProSpecT (Al-exon, Sunnyvale, CA, USA) and the Color Vue (Seradyn, Indianapolis, IN, USA) were compared for their ability to detect Cryptosporidium in 236 formalin-fixed stool specimens using the Merifluor C/G (Meridian, Cincinnati, OH, USA) stain as the reference method. The initial sensitivities of the ProSpecT and the Color Vue were 96.0% and 76.

Comparison of the Clearview Chlamydia, the PACE 2 assay, and culture for detection of Chlamydia trachomatis from cervical specimens in a low-prevalence population.

The Clearview Chlamydia assay (Wampole Laboratories, Cranbury, N.J.), the PACE 2 DNA probe assay (GenProbe, San Diego, Calif.

Comparison of different methods and cell lines for isolating measles virus.

Infectivity titers were determined for seven strains of measles virus by using various methods and cell lines. The use of B95-8 cells in a shell vial assay resulted in the highest infectivity titers. Our data suggest that B95-8 is the cell line of choice for the isolation of measles virus.

Degree and length of viremia in adults with measles.

Measles viremia is thought to peak at onset of rash and diminish rapidly over the subsequent 2-3 days. The length of viremia and the proportion of peripheral blood mononuclear cells (PBMC) infected during measles were investigated in 8 adults. Blood was obtained from 7 patients between days 2 and 4 of rash.