[The effect of muscle activity on the blood neutrophil system in rats].

The effect of muscular activity (MA) on the amount of different classes of leukocytes in the blood, concentrations of myeloperoxidase and lysozyme in the blood plasma, and neutrophils and phagocytosis parameters of these cells, were studied in rats. The MA resulted in a decrease of marker proteins (myeloperoxidase and lysozyme) concentration in neutrophils and an increase of their concentrations in the blood plasma. The neutrophil phagocytic activity decreased.

[Secretion of lysozyme by blood neutrophils during physical exertion].

The effect of physical exercise on the lysozyme release from the blood neutrophils was investigated. The lysozyme RIA was adopted to evaluate its concentration. The RIA main parameters developed on the basis of specific antiserum and 125I-lysozyme, were noted.

[Radioimmunologic analysis of steroids. Scientific and practical aspects].

Many-year experience of using authors' own systems for steroid radioimmunoassay (RIA) is summarized. Synthesis of natural and anabolic steroid derivatives and their conjugation with bovine serum albumin are considered. Schemes of rabbits immunization with steroid-protein conjugates, and the antisera characteristics are described.

[Radioimmunological assay of human lysozyme and effect of exertion on its blood level].

Radioimmunoassay (RIA) of human lysozyme was developed using 125I-lysozyme and specific antiserum to the enzyme. Titre of antibodies produced in the antiserum was as high as 1:81,000, affinity constant constituted 1.3.