Design and Synthesis of Thioglycosylated Monolignol Dual Probes for Bioimaging of Lignin Biosynthesis.

Lignin biosynthesis is a critical process that underpins plant structural integrity and defenses. Central to this pathway are monolignol glucosides (MLGs), whose role as intermediates remains debated. To elucidate MLGs' involvement, we developed thioglycosylated monolignol probes compatible with click chemistry for in situ visualization of lignin biosynthesis.

Diastereoselective Anomeric C(sp)-H Cyclization Towards the Design of New Cyclophane-Braced Glycopeptides.

Here we report a macrocyclization route towards the synthesis of glycophane peptides by a selective C-H arylation of the anomeric bond. This approach demonstrates the power of Pd-catalysis C-H activation to access unusual cyclic peptides.

Development of a New Personalized Molecular Test Based on Endometrial Receptivity and Maternal-Fetal Dialogue: Adhesio.

Successful embryo implantation relies on a receptive endometrium and a maternofetal dialogue. Abnormal receptivity is a common cause of implantation failure in assisted reproductive techniques. This study aimed to develop a novel transcriptomic-based diagnostic assay, Adhesio, for assessing endometrial receptivity and guiding personalized embryo transfer.

The Effects of Colchicine on Lipoprotein(a) and Oxidized Phospholipid Associated Cardiovascular Disease Risk.

Aims: Inflammatory lipoprotein(a) [Lp(a)] and oxidized phospholipids (OxPLs) on lipoproteins convey residual cardiovascular disease risk. The LoDoCo2 (low-dose colchicine 2) trial showed that colchicine reduced the risk for cardiovascular events occurring on standard therapies in patients with chronic coronary disease (CCS). We explored the effects of colchicine on Lp(a) and oxidized lipoprotein associated risk in a LoDoCo2 biomarker subpopulation.

Rapid Systematic Screening of Bispecific Antibody Surrogate Geometries for T-Cell Engagement Using DNA Nanotechnology.

Bispecific antibodies (bsAbs) are emerging immune-therapeutics, and many formats exist that differ considerably in structure. However, little systematic data exist about how the spatial organization of their components influences activity, requiring innovative approaches combining empirical and quantitative frameworks. This study presents a modular DNA nanotechnology platform to generate numerous bsAbs with surrogate geometries that span the structural features of the BiTE, IgG-like, and IgG-conjugate platforms to screen for T-cell engagement.