Analysis of oral lesion biopsies identified and evaluated by visual examination, chemiluminescence and toluidine blue.

Conventional visual examination and palpation remains the gold-standard for the identification of oral mucosal lesions. The purpose of this study was to investigate the adjunctive value of a chemiluminescent light source (ViziLite, Zila Pharmaceuticals, Phoenix, Arizona) and application of pharmaceutical grade toluidine blue (TBlue(630), Zila Pharmaceuticals, Phoenix, Arizona) to further assess lesions identified during the conventional oral soft tissue examination. Lesions deemed clinically suspicious by visual examination under incandescent light were further assessed under chemiluminescence and then application of toluidine blue stain.

The efficacy of oral lumenoscopy (ViziLite) in visualizing oral mucosal lesions.

Early diagnosis of oral mucosal lesions has been advocated as a means of improving outcomes of cancer therapy. Improved visualization of mucosal lesions may aid in diagnosis by guiding tissue sampling or referral. This multicenter study reports the effect of chemiluminescent light (ViziLite) upon visualization of mucosal lesions.

False-negative hybrid capture II results related to altered adhesion molecule distribution in women with atypical squamous cells pap smear results and tissue-based human papillomavirus-positive high-grade cervical intraepithelial neoplasia.

Objective: We investigated the detection of high-risk human papillomavirus DNA with the Hybrid Capture II test (HCII; Digene Corporation, Beltsville, MD) and the presence and pattern of distribution of adhesion molecules in biopsy-proven high-grade neoplastic lesions containing high-risk HPV-DNA from women with ASC cytology results.

Materials And Methods: We screened 4,600 women and performed colposcopy in 278 women with atypical squamous cells cytologic results. All women underwent HCII and tissue-based polymerase chain reaction analysis for high-risk HPV subtypes.

Transepithelial sampling of the uterine ectocervix with a stiff-bristled, spiral-shaped brush.

Objective: To measure the depth of sampling of the uterine ectocervix with the use of a stiff-bristled, spiral-shaped brush (SpiraBrush Cx; Trylon Corporation, Torrance, CA).

Materials And Methods: Eligible hysterectomy specimens were identified. The ectocervix was brushed with an inked SpiraBrush Cx in four quadrants with either light (gentle) or heavy (forceful) pressure.

A stiff bristled, spiral-shaped ectocervical brush: a device for transepithelial tissue biopsy.

Objective: To compare a new spiral-shaped tissue-sampling brush with a standard cervical punch biopsy.

Methods: Before large loop excision of the transformation zone, women with cervical intraepithelial neoplasia underwent a transepithelial brush biopsy of a portion of a colposcopically identified lesion, followed by a punch biopsy of the remaining portion. Brush biopsy samples were processed using liquid-based cytology and cell block techniques.