Significance of oxidative stress in liver damage of male and female rats with simulated ethanol hepatitis.

Objective: Aim: to find out the gender characteristics of oxidative mechanisms of liver damage in rats with ethanol hepatitis (EH).

Patients And Methods: Materials and Methods: The study was performed on 48 white male and female rats: 1 - control, 2 - acute EH (12,5 ml/kg of a 40% ethanol solution prepared on 5% solution of glucose for 7 days).The diene and triene conjugates (DC, TC), Schiff's bases (SB), TBA-active products (TBA-ap), activity of superoxide dismutase (SOD) and catalase (CAT) were determined in the blood serum.

Spectroscopically Orthogonal Spin Labels in Structural Biology at Physiological Temperatures.

Electron paramagnetic resonance spectroscopy (EPR) is mostly used in structural biology in conjunction with pulsed dipolar spectroscopy (PDS) methods to monitor interspin distances in biomacromolecules at cryogenic temperatures both in vitro and in cells. In this context, spectroscopically orthogonal spin labels were shown to increase the information content that can be gained per sample. Here, we exploit the characteristic properties of gadolinium and nitroxide spin labels at physiological temperatures to study side chain dynamics via continuous wave (cw) EPR at X band, surface water dynamics via Overhauser dynamic nuclear polarization at X band and short-range distances via cw EPR at high fields.

Isolation of a novel heterodimeric PSII complex via strep-tagged PsbO.

The multi-subunit membrane protein complex photosystem II (PSII) catalyzes the light-driven oxidation of water and with this the initial step of photosynthetic electron transport in plants, algae, and cyanobacteria. Its biogenesis is coordinated by a network of auxiliary proteins that facilitate the stepwise assembly of individual subunits and cofactors, forming various intermediate complexes until fully functional mature PSII is present at the end of the process. In the current study, we purified PSII complexes from a mutant line of the thermophilic cyanobacterium Thermosynechococcus vestitus BP-1 in which the extrinsic subunit PsbO, characteristic for active PSII, was fused with an N-terminal Twin-Strep-tag.

[Composition of oropharyngeal microbiota in patients with COVID-19 of different pneumonia severity].

Aim: To identify features of the taxonomic composition of the oropharyngeal microbiota of COVID-19 patients with different disease severity.

Materials And Methods: The study group included 156 patients hospitalized with confirmed diagnosis of COVID-19 in the clinical medical center of Yevdokimov Moscow State University of Medicine and Dentistry between April and June 2021. There were 77 patients with mild pneumonia according to CT (CT1) and 79 patients with moderate to severe pneumonia (CT2 and CT3).