Comprehensive Qualitative Drug Screening in Emergency Toxicology Using an Automated LC-MS System:Simultaneous Quantification of Relevant Drugs and Metabolites in Blood Plasma.

Rapid and comprehensive qualitative and quantitative analytical procedures are crucial in 24/7 emergency toxicology (ET) to support diagnosis and treatment of acute intoxications and to monitor their progression and efficacy of detoxification strategies. This study aimed to develop the simultaneous qualitative and quantitative analysis of 62 drugs, as well as seven active metabolites in human blood plasma using an automated liquid chromatography (LC)-linear ion trap mass spectrometry (MS) screening system. Sample preparation was conducted by liquid-liquid extraction, and plasma concentrations were determined using an electronically stored 5-point calibration.

Supine Traction vs Fulcrum Bending Radiographs in Preoperative Imaging of Scoliosis Patients Treated With Magnetically Controlled Growing Rods (MCGR) - which Technique is Better to Predict Surgical Correction of the Main Curve?

Study Design: Retrospective Cohort Study.

Objectives: Flexibility radiographs such as traction or bending radiographs are essential in preoperative imaging to assess for curve flexibility and to estimate the amount of operative correction in order to determine the type and length of instrumentation in growth-accompanying scoliosis treatment. Both traction and bending radiographs are controversially discussed in the literature.

AlkaPhos: a novel fluorescent probe as a potential point-of-care diagnostic tool to estimate recurrence risk of meningiomas.

Deletion of the short arm of chromosome 1 (1p) increases recurrence rates in meningiomas by up to 33%, regardless of tumor grade, correlating with absence of intracellular alkaline phosphatase enzyme activity. Current screening methods for 1p deletion like fluorescence in situ hybridization (FISH) and loss of heterozygosity (LOH) analysis are resource-intensive. This study evaluated AlkaPhos, a novel fluorescent probe, for detecting alkaline phosphatase in meningioma cells and compared findings with FISH, LOH, and histochemical analysis.

Evaluation of the lengthening of the magnetically controlled growing rods in juvenile and early-onset scoliosis.

Introduction: The treatment of early onset scoliosis (EOS) has seen significant advancements, particularly with the introduction of magnetically controlled growing rods (MCGR). However, a discrepancy between reported and actual rod lengthening during treatment has raised concerns.

Hypothesis: This retrospective study aims to investigate the disparity between reported and actual lengthening of MCGR and to identify factors contributing to the failure of the lengthening process.