Oocyte Cryopreservation: A 9-Year Single-Centre Experience.

Objective: Oocyte cryopreservation (OC) has increased in recent years; however, there is a paucity of published data on the use of cryopreserved oocytes and associated outcomes.

Methods: A retrospective review of 748 OC cycles between 2013 and 2022 at a private fertility centre was performed. Outcome parameters for oocyte retrieval cycles were reviewed.

Long-Term Effects of Simulated Microgravity and Vibration Exposure on Skeletal Development in Zebrafish.

Most studies utilizing fish to study the effects of simulated microgravity (SMG) only observe the effects during the first week of development. They also do not take into account the potential impact on development of vibrations caused by the equipment. In this study we analyze the effects of both SMG and vibration on development of the skeleton.

Comparison of Sexual Experience and Behavior between Bipolar Outpatients and Outpatients without Mood Disorders.

Sexual behavior over the past year of 32 outpatients with Bipolar disorder is compared to that of 44 Comparison patients that had never had an episode of affective illness. Subjects were outpatients treated with drugs and psychotherapy in routine office practice. Differences in sexual behavior between the two groups as a whole were minimal, but meaningful differences emerged when subgroups were compared.

An assessment of the long-term effects of simulated microgravity on cranial neural crest cells in zebrafish embryos with a focus on the adult skeleton.

It is becoming increasingly important to address the long-term effects of exposure to simulated microgravity as the potential for space tourism and life in space become prominent topics amongst the World's governments. There are several studies examining the effects of exposure to simulated microgravity on various developmental systems and in various organisms; however, few examine the effects beyond the juvenile stages. In this study, we expose zebrafish embryos to simulated microgravity starting at key stages associated with cranial neural crest cell migration.

A quick whole-mount staining protocol for bone deposition and resorption.

Osteoblasts and osteoclasts, two cell types important in bone development, are associated with enzymes capable of hydrolyzing phosphate groups. These enzymes are important for their function of bone deposition and resorption. Alkaline phosphatase (AP) activity is associated with the cell surface of osteoblasts, while osteoclasts secrete tartrate-resistant acid phosphatase (TRAP).