CEACAM5 overexpression is a reliable characteristic of CD133-positive colorectal cancer stem cells.

Background: CD133 (prominin-1) is the most commonly used molecular marker of the cancer stem cells (CSCs) that maintain tumor progression and recurrence in colorectal cancer. However, the proteome of CSCs directly isolated from colorectal tumors based on CD133 expression has never been investigated.

Objective: To reveal biomarkers of CD133-positive colorectal CSCs.

[Expression of ganglioside GD2 on colorectal adenocarcinoma cells].

Using flow cytometry GD2 ganglioside expression was evaluated both on colorectal adenocarcinoma cell lines and on tumor tissue samples from colorectal cancer patients. The marker was found on EpCAM-positive tumor cells in 6 of 12 patients' samples but not on the HT29 and CaCo-2 cell lines. GD2 expression was not an exceptional feature of cancer stem cells, since its expression level was similar on CD133-positive and CD133-negative tumor cells.

[Pancreatoduodenectomy: learning curve within single multi-field center].

Aim: To analyze learning curve by using of immediate results of pancreatoduodenectomy at multi-field oncology institute.

Material And Methods: For the period 2010-2016 at Abdominal Oncology Department of Herzen Moscow Oncology Research Institute 120 pancreatoduodenal resections were consistently performed. All patients were divided into two groups: the first 60 procedures (group A) and subsequent 60 operations (group B).