[ThE COMPARATIVE CHARACTERISTIC OF PHENOTYPIC AND GENOTYPIC RESISTANCE TO AMINOGLYCOSIDES OF STRAINS STAPHYLOCOCCUS AUREUS ISOLATED IN TRAUMATOLOGICAI ORTHOPEDIC HOSPITAL].

The clinical isolates of Staphylococcus aureus (n = 102) were analyzed on sensitivity and to gentamicin, tobramicin, netimicin and amikacin. The disc diffusing technique was applied. The technique ofpolymerase chain reaction was applied to analyze all strains establishing presence in their genomes genes aac (6'-Ie/aph(2"), ant1, aac, ant(6)-Ia, aph (3')-IIIa and ant(4')-Ia coding amino-glycoside-modifying enzymes.

A new antibiotic, TH818, and its properties.

Antibiotic TH818 was isolated and purified from a culture broth of Streptomyces fulvoviolaceus 818 by extraction and reversed-phase liquid chromatography. The antibiotic TH818 possesses high molecular mass and unique structure, constructed from carbohydrates, amino acids and lipids. The hydrolysis experiments showed the presence of six fatty acids (hexadecanoic, hexadecenoic, octadecanoic, iso-nonadecanoic, docosanoic and pentacosanoic acids), four sugars (glucose, galactose, N -acetylglucosamine and N -acetylgalactosamine), and seven amino acids (threonine, alanine, glutamic acid, glycine, serine and two unidentified).

Fatty acid composition of Streptomyces hygroscopicus strains producing antibiotics.

The fatty acid composition from mycelia of Streptomyces hygroscopicus strains was studied. A significant proportion of C18:2 was found in cultures. High levels of C16:0, iso-C18:0 and C18:1 were also detected in all S.