Adhesion and differentiation behaviors of mesenchymal stem cells on titanium with micrometer and nanometer-scale grid patterns produced by femtosecond laser irradiation.

To clarify the effects of grid topographies with different scales on cell morphology and functionalization, we investigated the adhesion and differentiation of human mesenchymal stem cells (hMSCs) to titanium surfaces with micron, nano, and micron/nano (hybrid) grid topographies created by femtosecond laser irradiation. The results showed that cellular adhesion and differentiation strongly depended on the scales of the grid topography. hMSCs cultured on micron and hybrid grid topographies showed regulation of cellular adhesion plaques following the surface topography and were vinculin-positive, whereas filamentous vinculin was evident at the filopodia of hMSCs cultured on nanogrids.

Micron/Submicron Hybrid Topography of Titanium Surfaces Influences Adhesion and Differentiation Behaviors of the Mesenchymal Stem Cells.

To clarify the effects of micron/submicron hybrid topography on cell morphology and functionalization, we investigated the adhesion and differentiation of human mesenchymal stem cells (hMSCs) to titanium (Ti) surfaces with three different topographies: micron, submicron, and hybrid grooves created using a femtosecond laser. hMSCs cultured on Ti specimens showed high alignment on micron and hybrid surfaces after 6 h of incubation, whereas cells attached to submicron and hybrid surfaces were elongated. An examination of vinculin-positive adhesion plaques indicated that micron grooves affected cellular alignment by modifying the initial cell polarization, whereas submicron grooves affected cellular extension.

Serum antioxidants and subsequent mortality rates of all causes or cancer among rural Japanese inhabitants.

The purpose of this study was to investigate whether a relationship exists between mortality rates and serum antioxidant levels among Japanese inhabitants. The follow-up subjects, who participated in comprehensive health examinations, consisted of 2444 inhabitants (949 males and 1495 females) of a rural area in Hokkaido, Japan. Between 1991 and December 2000, 146 subjects (94 males and 52 females) died, with cancer accounting for 76 of these deaths (48 males and 28 females).

Mortality rates from cancer or all causes and SOD activity level and Zn/Cu ratio in peripheral blood: population-based follow-up study.

A total of 507 residents (232 males and 275 females) of a rural area of Hokkaido, Japan, were enrolled in the present follow-up study as the follow-up cohort. We investigated the relationship between mortality rates from cancer or all causes and blood levels of SOD activity and Zn/Cu ratio, and serum levels of SOD activity and lipid peroxides (TBARS), by following up our subjects over the course of 18 years. The hazard ratios of lowest blood levels of SOD activity and Zn/Cu ratios to mortality rates from cancer were 1.