[Contribution of indicators of sensitized T lymphocytes in the diagnosis of IgE independent and IgE dependent cow's allergy in children].

Background: Food allergy is an abnormal response of the immunological system, especially of mucosal immunological system on antigens supplied per os. There are very complicated and still unexplained immunological mechanisms, which lead to hypersensitivity reaction. Most often food hypersensitivity is identified as the effect of atopy, which is connected with humoral response (specific IgE antibody).

[Assessment of the correlation between proliferation defined by S-phase cell cycle and apoptosis after preoperative chemotherapy in osteosarcomas of children and adolescents].

Estimation of the S-phase of the cell cycle is applied to indicate the rate of cancer cells proliferation. This information is clinically useful, especially with the context of apoptosis (programmed cell death) stimulated by anticancer agents. The presented studies concerned above problems in children and adolescents osteosarcomas after preoperative chemotherapy.

[Cytometric assessment of apoptosis and aneuploidy in relation to survival time in osteosarcomas of children and adolescents].

Apoptosis programmed cell death takes place in response to many different stimuli, which include DNA damage. This is interest for therapeutic and prognostic purposes in neoplasms. Laboratory studies have shown that many anti-cancer agents (including cytostatics) achieve their cytotoxic effect by inducing apoptosis.

[Cytometric analysis of the relation between aneuploidy and expression of receptors for insulin growth factor I in child and adolescent osteosarcoma, after preliminary chemotherapy].

Introduction: Aneuploidy cell population is often seen as a marker of malignancy. The influence of growth factors on this cell population is of interest for oncologists, in the context of the clinical course of disease and the results of therapy.

Aim: To investigate of reciprocal relations between aneuploidy parameters and expression of receptors of insulin-like growth factor I (IGF-IR) in osteosarcomas after preliminary chemotherapy.

[Relationship between the expression of insulin-like growth factor I receptors and apoptosis after preliminary chemotherapy, in child and adolescent osteosarcoma].

Introduction: The influence of growth factors and apoptosis on the clinical course of neoplasms is of significant interest for oncologists, especially neoplasms with dynamic changes in proliferation, such as child and adolescent osteosarcoma.

Aim: To investigate reciprocal relations between the expression of insulin-like growth factor I receptors (IGF-IR) and index apoptosis ("pre-G l /G 0" fraction, DNA histograms) in osteosarcomas before operation and after preliminary chemotherapy.

Material And Methods: Flow cytometric analysis was carried out in 20 children and adolescents with osteosarcoma (aged 6 to 18 years) after preliminary chemotherapy.

[CD69 and PCNA expression of T-lymphocytes from children with cow's milk allergy (CMA)].

Background: As the frequency of allergic disease and especially the food allergy in children is still increasing - we undertook studies to determine the specific sensitivity of lymphocytes T in order to improve the diagnostics and monitoring of treatment. The usefulness of evaluation CD69 and PCNA expression of T cells from children with cow's milk allergy (CMA) in these procedures was examined.

Aim: The study on usefulness of lymphocytes CD69 and PCNA detection for diagnosis of CMA in children and for monitoring of the treatment.

[Flow cytometry estimate of s phase cell cycle and prognosis of osteosarcoma in children and adolescents].

Flow cytometry analysis has been used to measure the S phase in paraffin embedded osteosarcoma tissues of 22 children and adolescents who were surgically treated. The aim of this study was the assessment of S phase cell cycle in relation to survival, localization of tumour and histopathological grading. Our findings demonstrate the usefulness of flow cytometry determination of S phase cell cycle as a prognostic factor.

[Clinical characteristics of osteosarcomas and cell aneuploidy in children and adolescents -- retrospective studies].

The relationship between the clinical status skeletal involvement, frequency of metastases and histopathological grading of osteosarcomas 18 children and adolescents and abnormal DNA content (aneuploidy) was analysed. Our findings demonstrate a correlation between Index DNA (DI), percentage of aneuploidy population to histopathological grading and prognosis. No significant correlation was found between aneuploidy and the site of skeletal involvement.

[Aneuploidy and histopathological classification, according to the Musculoskeletal Tumour Society and WHO, of osteosarcoma in children and adolescents].

A flow cytometry analysis in children and adolescents with osteosarcoma was carried out. Investigation concerned 32 fresh surgical specimens (from patients after preoperative chemotherapy) and 18 paraffin-embedded tumour tissues (archive material). The correlation of aneuploidy population parameters to malignancy grading acc.

[Analysis of DNA histograms and histopathologic assessment of necrosis after preoperative chemotherapy of osteosarcoma in children and youth].

Analysis of nucler DNA content (DNA histograms by flow cytometry) in 26 children and youth with osteosarcoma treated at the National Research Institute of Mother and Child, was carried out. The relationship between aneuploid populations, index DNA and phases of cell cycle and the clinical course, histopathologic grading and histopathologic assessment of preoperative chemotherapy effect (% of tumour necrosis) were analysed. Osteosarcomas in children with changed schedules of chemotherapy (therapy complications) in relation to the above parameters were also examined.

[Value of comparing flow cytometry examination and histopathology in prognosing of osteogenic sarcoma - preliminary report].

Our preliminary report presents two cases of osteogenic sarcoma examined after initial chemotherapy. In both cases histological maps with morphological effects of chemotherapy and flow cytometry of DNA content in tumour cells were made. Results of flow cytometric DNA histograms were linked with an estimation of the proliferative potential of tumours.

[The effect of tobacco smoking on the human immune system].

The influence of tobacco smoking in development of respiratory, cardiovascular diseases and impairment of foetal development is well documented. Recent studies have shown adverse effects of tobacco smoke on the immune system. Particular place of impact of tobacco smoke is BALT (bronchus-associated lymphoid tissue).

[Flow cytometry, basic principles and applications--with special reference to pediatrics].

Flow cytometry in clinical applications and research is the result of knowledge and techniques concerning cyto and fluorochemistry, laser technology, monoclonal antibodies and computer processing. Flow cytometry can assess multiple physical and biological cell properties, in a relatively short period of time. It is specially important, that specimens can concern different types of material: fresh solid tissue, embedded in paraffin, blood, bone marrow, body fluids.

[The place and role of health promotion in oncology].

At the present time, groups people who live with chronic illness or outcome of medical intervention eliminated the disease process are in greater and greater number. It concerns cancer-patients and their life- complication too. Innovative approaches towards to health promotion in this field is actual and important.

[Anemia and recurrences in acute lymphoblastic leukemia in children after the treatment and the frequency of the erythroblasts with micronuclei].

A retrospective analysis of the relation of relapses and anaemias to the frequency of micronucleated erythroblasts in bone marrow smear in 140 children with acute lymphoblastic leukaemia after BFM and Memphis schemes of therapy was carried out. The anaemias correlated with the frequency of micronucleated erythroblasts in statistically significant manner. Correlation concerning relapses, was not shown.

[Selected immunologic parameters and infections in children in over 1 year after completion of the treatment of acute lymphoblastic leukemia].

An analysis of infection incidence within one year after completion of the treatment for the acute lymphoblastic leukemia was performed in the group of 24 children. Infections incidence was related to the selected immunological parameters, mainly T4 and T8 lymphocyte subpopulations assayed with monoclonal antibodies. T4/T8 cells ratio (Ix) was determined.

[Study of expression of surface antigen p72 using monoclonal antibody 79IT/36 and the effect of ricin A chain-bound 79IT/36 on phytohemagglutinin-stimulated human lymphocytes].

Monoclonal antibody 791T/36 directed against surface antigen on the human osteosarcoma cell line and cross-reacting with surface antigen p72 presenting on human PHA-stimulated T-lymphoblasts was used in analysis of p72 antigen expression on human mononuclear peripheral blood cells, between 2-4 days of culture. Using FACS-IV system and fluorescence microscope, it was shown that expression of p72-antigen is dependent on PHA-stimulation and the ability of lymphocytes proliferation. The expression of p72 preceding the entry of the cells into the cell cycle.

Cross-reaction of 791T/36 monoclonal antibody with immunological activated human peripheral blood mononuclear cells.

Monoclonal antibody 791T/36, directed against surface antigen on the human osteosarcoma cell line (791T), and cross-reacting with surface antigen p72 expressed on activated (PHA, MLR) human peripheral blood mononuclear cells (PBM), was used in analysis of p72 antigen in vitro. Using FACS-IV system and fluorescence microscope, it was shown that expression of p72 antigen on PBM cells is dependent on phytohaemagglutinin (PHA) stimulation, and alloantigens in mixed lymphocyte reaction (MLR). The increase of p72 expression is always connected with lymphocytes proliferation activity (FACS-IV analysis and 3H-Thymidine incorporation assay).

Analysis of surface antigen expression per cell of human osteogenic sarcoma cells by fluorescence-labelled monoclonal antibodies.

The relationship between total surface antigen expression per cell (means) - measured by fluorescence-labelled monoclonal antibodies (fluorescence-histograms) and the distribution of cells in the cell cycle (DNA-histograms) and size-scattergrams (cell sorter FACS-IV) were analysed in drug treated unsynchronized and synchronized osteogenic sarcoma cells (2OS) in vitro. Drugs with various sites of action in the cell cycle were used. Adriamycin, Vindesine, in concentrations applied accumulate cells in G2 + M phase.