Publications by authors named "Ryoya Sekine"
Article Synopsis
- * The study focused on ALDH1L1, an enzyme that breaks down 10-fTHF, which is often less active in hepatocellular carcinoma (HCC), leading to altered serine and glycine levels and increased accumulation of a dnPS intermediate called ZMP.
- * HCC cells with higher ALDH1L1 expression showed decreased sensitivity to ZMP and better mitochondrial function, suggesting that low ALDH1L1 levels could make cells more responsive
The interaction of the β-coronavirus severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) nucleocapsid (N) protein with genomic RNA is initiated by specific RNA regions and subsequently induces the formation of a continuous polymer with characteristic structural units for viral formation. We hypothesized that oligomeric RNAs, whose sequences are absent in the 29.9-kb genome sequence of SARS-CoV-2, might affect RNA-N protein interactions.
View Article and Find Full Text PDFCRISPR/Cas9 has emerged in various organisms as a powerful technology for targeted gene knockout; however, no reports of editing the Dictyostelium genome efficiently using this system are available. We describe here the application of CRISPR/Cas9-mediated gene modification in Dictyostelium. The endogenous tRNA-processing system for expressing sgRNA was approximately 10 times more effective than the commonly used U6 promoter.
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