Publications by authors named "Ryosuke Kashiwagi"
Article Synopsis
- Liquid biopsy (LB) is a minimally invasive method for analyzing tumor-derived materials, and this study explores the potential of bile as a superior source of cell-free DNA (cfDNA) compared to plasma for detecting mutations in biliary tract cancers.
- The research involved collecting cfDNA from the bile of 24 cancer cases, including various types of biliary tract cancers, and applying a multigene panel to identify cancer driver mutations.
- Results indicated that bile yielded a higher detection rate of cancer driver mutations, with 54% of bile samples testing positive compared to just 17% of plasma samples, highlighting bile's effectiveness for liquid biopsy analysis.
Background: Splenectomy is indicated in cases of autoimmune hemolytic anemia (AIHA), which are refractory to medical management. In post-splenectomy, there exists a theoretical risk of AIHA recurrence, especially if an accessory spleen undergoes compensatory hypertrophy. In this context, we present a unique case of recurrent AIHA managed through laparoscopic excision of the accessory spleen (LEAS).
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- ABCC3 (MRP3) is a transporter for bile acids that is downregulated in colorectal cancer due to the Wnt/β-catenin pathway, raising questions about its role in cancer development.
- * Research found that genes associated with bile acid secretion, including ABCC3, were already downregulated during the early stages of cancer, like adenoma formation.
- * Manipulating ABCC3 levels affected the concentration of deoxycholic acid in cancer cells, influencing MAPK signaling, and nonsteroidal anti-inflammatory drugs were shown to increase ABCC3 expression, indicating its potential as a therapeutic target in colorectal cancer.*
Expression of the gene for collagen XVII (COL17A1) in tumor tissue is positively or negatively associated with patient survival depending on cancer type. High COL17A1 expression is thus a favorable prognostic marker for breast cancer but unfavorable for pancreatic cancer. This study explored the effects of COL17A1 expression on pancreatic tumor growth and their underlying mechanisms.
View Article and Find Full Text PDFPancreatic fistula is a potentially morbid complication after distal pancreatectomy. Chronic glucocorticoid use is one of the risk factors for pancreatic fistula in pancreaticoduodenectomy, though it has not been reported in distal pancreatectomy. We explored whether chronic glucocorticoid use can be a risk factor for pancreatic fistula in distal pancreatectomy.
View Article and Find Full Text PDFPurpose: We investigated the characteristics of hyperpolarized (HP) (129)Xe magnetic resonance (MR) imaging obtained from balanced steady-state free precession (SSFP) measurement of mouse lungs, especially under spontaneous breathing, and compared the results with those obtained using traditional spoiled gradient echo (SPGR) method, focusing on improved signal-to-noise ratio (SNR) and reduced total acquisition time.
Methods: We calculated magnetization response of the HP (129)Xe gas for the balanced SSFP sequence under spontaneous breathing to derive optimal conditions for the imaging experiment. We then placed an anesthetized mouse in the magnet (9.