Multimass Analysis of Adeno-Associated Virus Vectors by Orbitrap-Based Charge Detection Mass Spectrometry.

Adeno-associated virus (AAV) vectors have attracted significant attention as the main platform for gene therapy. To ensure the safety and efficacy of AAV vectors when used as gene therapy drugs, it is essential to assess their critical quality attributes (CQAs). These CQAs include the genome packaging status, the size of the genome encapsidated within the AAV capsid, and the stoichiometry of viral proteins (VPs) that constitute the AAV capsids.

Synchronized resistive-pulse analysis with flow visualization for single micro- and nanoscale objects driven by optical vortex in double orifice.

Resistive-pulse analysis is a powerful tool for identifying micro- and nanoscale objects. For low-concentration specimens, the pulse responses are rare, and it is difficult to obtain a sufficient number of electrical waveforms to clearly characterize the targets and reduce noise. In this study, we conducted a periodic resistive-pulse analysis using an optical vortex and a double orifice, which repetitively senses a single micro- or nanoscale target particle with a diameter ranging from 700 nm to 2 [Formula: see text]m.

Effect of hydrodynamic inter-particle interaction on the orbital motion of dielectric nanoparticles driven by an optical vortex.

We experimentally and theoretically characterize dielectric nano- and microparticle orbital motion induced by an optical vortex of the Laguerre-Gaussian beam. The key to stable orbiting of dielectric nanoparticles is hydrodynamic inter-particle interaction and microscale confinement of slit-like fluidic channels. As the number of particles in the orbit increases, the hydrodynamic inter-particle interaction accelerates orbital motion to overcome the inherent thermal fluctuation.