Publications by authors named "Ryohei Fukuda"
Article Synopsis
- The enzyme S-hydroxymethylglutathione dehydrogenase from the fungus Paecilomyces variotii was purified using various methods, achieving a purification factor of 122 with a yield of 10.5%.
- It has a molecular mass of about 49 kDa, exhibits optimal activity at pH 8.0 and 40°C, and is especially specific for formaldehyde as a substrate, with limited activity for pyruvaldehyde.
- The enzyme's activity is positively influenced by certain divalent cations like Mn2+ and Ba2+, but inhibited by metals like Ni2+ and Hg2+, indicating that the enzyme's sulfhyd
