Publications by authors named "Ryoga Tsurigami"
Article Synopsis
- DNA-binding transcription factors, like KojR, are proteins that attach to specific DNA sequences to control gene expression, specifically in kojic acid metabolism.
- The study employed techniques like gSELEX-Seq and RNA-Seq to identify KojR-associated genes and confirmed that KojR binds preferentially to a specific DNA sequence.
- Additionally, KojR is suggested to regulate genes related to the carbon metabolic pathway, emphasizing its significant role in carbon metabolism in the organism studied.
A comprehensive analysis to survey heme-binding proteins produced by the white-rot fungus Phanerochaete chrysosporium was achieved using a biotinylated heme-streptavidin beads system. Mitochondrial citrate synthase (PcCS), glyceraldehyde 3-phosphate dehydrogenase (PcGAPDH), and 2-Cys thioredoxin peroxidase (mammalian HBP23 homolog) were identified as putative heme-binding proteins. Among these, PcCS and PcGAPDH were further characterized using heterologously expressed recombinant proteins.
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