Infrared spectroscopy analysis determining secondary structure change in albumin by cerium oxide nanoparticles.

Cerium oxide (CeO) nanoparticles are expected to have applications in the biomedical field because of their antioxidative properties. Inorganic nanoparticles interact with proteins at the nanoparticle surface and change their conformation when administered; however, the principle underlying this interaction is still unclear. This study aimed to investigate the secondary structural changes occurring in bovine serum albumin (BSA) mixed with CeO nanoparticles having different surface modifications using Fourier transform infrared spectroscopy.

Changes in the Secondary Structure and Assembly of Proteins on Fluoride Ceramic (CeF) Nanoparticle Surfaces.

Fluoride nanoparticles (NPs) are materials utilized in the biomedical field for applications including imaging of the brain. Their interactions with biological systems and molecules are being investigated, but the mechanism underlying these interactions remains unclear. We focused on possible changes in the secondary structure and aggregation state of proteins on the surface of NPs and investigated the principle underlying the changes using the amyloid β peptide (Aβ) based on infrared spectrometry.