Optimization of β-Fructofuranosidase Production from Agrowaste by and Its Application in the Production of Inverted Sugar.

Research Background: Microbial β-fructofuranosidases are widely employed in food industry to produce inverted sugar or fructooligosaccharides. In this study, a newly isolated PC-4 strain was used to optimize the β-fructofuranosidase production in a cost-effective process and the sucrose hydrolysis was evaluated to produce inverted sugars.

Experimental Approach: Optimization of nutritional components of culture medium was carried out using simplex lattice mixture design for 72 and 120 h at 28 °C.

-Fructofuranosidase and -D-Fructosyltransferase from New PC-4 Strain Isolated from Canned Peach Syrup: Effect of Carbon and Nitrogen Sources on Enzyme Production.

-fructofuranosidase (invertase) and -D-fructosyltransferase (FTase) are enzymes used in industrial processes to hydrolyze sucrose aiming to produce inverted sugar syrup or fructooligosaccharides. In this work, a black sp. PC-4 was selected among six filamentous fungi isolated from canned peach syrup which were initially screened for invertase production.