Experimental study of the liver ultrastructure in the presence of infection with herpes simplex virus 1.

Objective: Introduction: Understanding of HSV-1liver infection pathogenesis is of great scientific, social and economic significance, since this is one of the main latent infections in population. However reactivation of this infection remains understudied. The aim: This experimental research aimed at studying the ultrastructure changes occurring in the liver in the presence of HSV-1infection.

Complexes of Oligoribonucleotides with d-Mannitol Modulate the Innate Immune Response to Influenza A Virus H1N1 (A/FM/1/47) In Vivo.

Rapid replication of the influenza A virus and lung tissue damage caused by exaggerated pro-inflammatory host immune responses lead to numerous deaths. Therefore, novel therapeutic agents that have anti-influenza activities and attenuate excessive pro-inflammatory responses that are induced by an influenza virus infection are needed. Oligoribonucleotides-d-mannitol (ORNs-d-M) complexes possess both antiviral and anti-inflammatory activities.

Nerve Regeneration in Conditions of HSV-Infection and an Antiviral Drug Influence.

Herpes simplex virus type I (HSV-I) is a latent neuroinfection which can cause focal brain lesion. The role of HSV-infection in nerve regeneration has not been studied so far. The aim of the work was to study sciatic nerve regeneration in the presence of HSV-infection and the influence of an antiviral drug.

The study of leukocyte phagocytic activity in the presence of herpetic infection and stroke.

Objective: Inrtoduction: Post-stroke complications are one of the urgent and insufficiently resolved problems. According to different literature data 23% to 65% of patients suffer from the post-stroke development of an infectious process. Herpes simplex virus type 1 and 2 can also be etiological factors of stroke development, however their reactivation is seldom mentioned in clinical observations.

[Glibenclamide as a promising agent for prevention and treatment of cerebral edema].

The article presents a review of the literature on the use of a fundamentally new technique for prevention and treatment of cerebral edema. A drug glibenclamide, which is used to treat type 2 diabetes mellitus, is able to reduce cerebral edema and neuronal damage as evidenced by the results of preclinical trials in rodents and the first results of drug application in patients. The article describes the mechanism of glibenclamide action and discusses the potential for its application.

Anti-influenza Activity of a Bacillus subtilis Probiotic Strain.

Among bacteria, is the species that produces the most antimicrobial compounds. In this study, we analyzed the activity of probiotic strain 3 against the influenza virus. The antiviral effect of this strain has been demonstrated and A new peptide, P18, produced by the probiotic strain was isolated, purified, chemically synthesized, and characterized.

[Characterization of the Lipopolysaccharides of Pseudomonas chlororaphis].

Lipopolysaccharides (LPS) from two strains ot Pseudomonas chlororaphis subsp. aureofaciens,UCM B-111 and UCM B-306, were isolated and characterized. The LPS preparations exhibited low toxicity, high pyrogenicity and high antiviral activity.

[Morphological structure of rat epiphysis exposed to electromagnetic radiation from communication devices].

Pineal gland is one of the most important components of homeostasis - the supporting system of the body. It participates in the launch of stress responses, restriction of their development, prevention of adverse effects on the body. There was proved an impact of electromagnetic radiation on the epiphysis.

Antiviral activity of Basidiomycete mycelia against influenza type A (serotype H1N1) and herpes simplex virus type 2 in cell culture.

In this study, we investigated the in vitro antiviral activity of the mycelia of higher mushrooms against influenza virus type A (serotype H1N1) and herpes simplex virus type 2 (HSV-2), strain BH. All 10 investigated mushroom species inhibited the reproduction of influenza virus strain A/FM/1/47 (H1N1) in MDCK cells reducing the infectious titer by 2.0-6.

Photonic nonlinear transient computing with multiple-delay wavelength dynamics.

We report on the experimental demonstration of a hybrid optoelectronic neuromorphic computer based on a complex nonlinear wavelength dynamics including multiple delayed feedbacks with randomly defined weights. This neuromorphic approach is based on a new paradigm of a brain-inspired computational unit, intrinsically differing from Turing machines. This recent paradigm consists in expanding the input information to be processed into a higher dimensional phase space, through the nonlinear transient response of a complex dynamics excited by the input information.

[Influence of chemically different antiviral substances on the expression of IFNα, PKR, OAS1a and RNAse L genes].

The aim of the study was to determine the ability of several chemically different antiviral substances to induce the expression of interferon α(IFNα), PKR, OAS1a and RNAse L genes in the rat liver. The investigated substances included Amizon, Altabor and Proteflasid, which are already used in practical medicine, and 3',7-dimethylquercetin extracted from Proteflasid, the mixture of synthesized trimethyl- and tetramethylquercetin and Sialospecific lectin from persimmon, which are at the stage of preclinical trial and experimental research respectively. The content of corresponding mRNAs in total RNA was detected with the help of reverse transcription and polymerase chain reaction in real time.

Intermittency as a universal characteristic of the complete chromosome DNA sequences of eukaryotes: from protozoa to human genomes.

Large-scale dynamical properties of complete chromosome DNA sequences of eukaryotes are considered. Using the proposed deterministic models with intermittency and symbolic dynamics we describe a wide spectrum of large-scale patterns inherent in these sequences, such as segmental duplications, tandem repeats, and other complex sequence structures. It is shown that the recently discovered gene number balance on the strands is not of a random nature, and certain subsystems of a complete chromosome DNA sequence exhibit the properties of deterministic chaos.

[Anti-HIV effect of soy products and possibility of their usage as medicinal and prophylactic nutrition].

An anti-HIV effect of soy products (emulsion, flour) has been revealed in vitro experiment. The unique properties of biologically active components of soy products made from the whole soybeans justify their usage in HIV/AIDS treatment as an additional treatment and prophylactic nutrition.

Hidden chromosome symmetry: in silico transformation reveals symmetry in 2D DNA walk trajectories of 671 chromosomes.

Maps of 2D DNA walk of 671 examined chromosomes show composition complexity change from symmetrical half-turn in bacteria to pseudo-random trajectories in archaea, fungi and humans. In silico transformation of gene order and strand position returns most of the analyzed chromosomes to a symmetrical bacterial-like state with one transition point. The transformed chromosomal sequences also reveal remarkable segmental compositional symmetry between regions from different strands located equidistantly from the transition point.

[The role of the interferon system in pathogenesis of endothelial dysfunction in patients with metabolic syndrome].

We have studied the serum interferon activity and its relation to the endothelial dysfunction. Atherosclerosis development in patients with metabolic syndrome is followed by significant increase in interferon activity. Close relation presents between activity of the serum interferon and indexes of structural and functional changes of arterial vessels compromised with atherosclerosis process.

[Effect of probiotic lactic acid bacteria strains on virus infection].

The effect of probiotic lactic acid bacteria strains on viral infection was studied in experiments in vivo. It was shown that in investigations microbial substance based on living cells of L. plantarum and S.

[Reversion of antibiotic-sensitivity of lactic acid bacteria in grafted cultures of lymphoblastoid human cells].

The reversion of the antibiotic sensitivity of lactic acid bacteria: Streptococcus salivarius subsp. thermophilus, Enterococcus faecium, Lactobacillus plantarum have been studied. The phenomenon of the reversion has been fists studied in lactic acid bacteria of different taxonomic groups at their interactions with lymphoblastoid human cells.

[Interferon induction by lacticacid bacteria in vitro and in vivo experiments].

Interferonogenic acitivity of lacticacid bacteria was studied in experiments in vitro and in vivo. It have been shown, that interferon induction depends both on strain species and on culture medium used for lactobacteria harvesting. Streptococcus thermophilus induces interferon more actively in leucocytes (in vitro)--1280 AU/ml on the bines broth medium, and less actively--320 AU/ml on fermented corn medium.

Characteristics of extracellular DNA containing unmethylated CpG motifs isolated from Bacillus subtilis culture medium filtrate.

Unlabelled: The application of CpG DNA is one of the most modern and sufficiently perspective trends in immunotherapy of cancer. The AIM of the investigation was to characterize the DNA as a part of nucleoprotein fraction isolated from Bacillus subtilis culture medium filtrate (CMF) and to evaluate the dynamic of accumulation of such DNA and level of its unmethylated CpG motifs enrichment in the process of cultivation of bacterium.

Methods: Two bacterial strains--B.

Forward and backward laser-guided motion of an oil droplet.

Directed motion of an oil droplet floating in an aqueous solution is generated by using a laser beam. Interestingly, the direction of the droplet motion can be switched between forward and backward by changing the optical path of the laser through the droplet. This motion is caused above a certain critical power of the laser, and above this value the velocity increases almost linearly with the power.

[Dynamics of the excitable media with two interacting pacemakers].

A general model of the nonlinear interaction of two impulse systems was developed, which describes some types of cardiac arrhythmias. Phase locking phenomena were studied with consideration for refractoriness time. The phenomena of tongue splitting and the overlapping of the major synchronization regions were revealed.

[Construction of the immunoenzyme test system "IFA-AtEBV-Strip" for detecting antibodies of the Epstein-Barr virus].

The first home immunoenzyme test-system for finding antibodies to the Epstein-Barr virus (EBV) has been created. The test-system laboratory prototype was called "IFA-AtEBV-strip". Production technology has been developed for specific components of immunoenzyme test system and all the analysis stage conditions have been optimized: the antigen sorption on plates, its interaction with the studied serum, finding out of the formed immune complex.

Virus-inhibitory effect of a yeast RNA-tilorone molecular complex in cell cultures.

The virus-inhibitory activity of a molecular complex (MC) of tilorone and yeast RNA was studied in vitro on three virus-cell systems: vesicular stomatitis virus (VSV) - murine fibroblast L929 cells, Venezuelan equine encephalittis virus (VEEV) - swine embryo kidney (SEK) cells and encephalomyocarditis virus (EMCV) - established piglet testicular (EPT) cells. In all these systems the MC exerted an antiviral effect similar to that of polynucleotide interferon (IFN) inducers such as poly(I)-poly(C), larifan and ridostin. The antiviral effect of the MC was similar when the compound was applied before or after virus adsorption to cells.

6-azacytidine--compound with wide spectrum of antiviral activity.

6-azacytidine demonstrates activity against adenoviruses types 1, 2, 5. It inhibit synthesis of viral DNA and proteins. 6-AC shows antiherpetic and antiinfluenza action during experimental infection in mice.

Therapeutical effect of modified adamantane copolymer compounds: study of molecular mechanisms.

Copolymers of N-polyvinylpyrrolidone-acrylic acid (AB-1) and adamantane derivatives are known to possess marked antiviral activity in in vitro and in ovo models. Among the constructed preparations of AB-1 modified by adamantane derivatives some, especially AB-4 (modified by deitiforin), were found to show more extended antiviral activity and to inhibit markedly virus reproduction in susceptible permissive cell cultures and chicken embryos. In AB-4 treated cells and allantoic sacs, virus titers (influenza virus, herpes virus, and HIV) and virus antigen concentration were decreased.