Publications by authors named "Ryan Wimbles"

This paper presents a microfluidic device capable of performing genetic analysis on dung samples to identify White Rhinoceros (). The development of a microfluidic device, which can be used in the field, offers a portable and cost-effective solution for DNA analysis and species identification to aid conservation efforts. Optimization of the DNA extraction processes produced equivalent yields compared to conventional kit-based methods within just 5 minutes.

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FTA paper can be used to protect a variety of biological samples prior to analysis, facilitating ease-of-transport to laboratories or long-term archive storage. The use of FTA paper as a solid phase eradicates the need to elute the nucleic acids from the matrix prior to DNA amplification, enabling both DNA purification and polymerase chain reaction (PCR)-based DNA amplification to be performed in a single chamber on the microfluidic device. A disc of FTA paper, containing a biological sample, was placed within the microfluidic device on top of wax-encapsulated DNA amplification reagents.

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