antiviral activity of NanB bacterial sialidase against avian influenza H9N2 virus in MDCK cells.

The avian influenza virus is an infectious agent that may cause global health problems in poultry and is potentially zoonotic. In the recent decades, bacterial-derived sialidases have been extensively studied for their ability to inhibit avian influenza virus infections. In this study, the antiviral activity of NanB sialidase from was investigated through analysis using Madin-Darby canine kidney (MDCK) cells.

sialidase interaction with Neu5Ac α-Gal sialic acid receptors by observation and its impact on monolayers cellular behavior structure.

Objective: This study aims to evaluate the effect of sialidase treatment on monolayer cell behavior using computational screening and an approach to demonstrate interaction between enzyme-based drugs and ligands in host cells.

Materials And Methods: The study was carried out by molecular docking analysis used to predict the interactions between atoms that occur, followed by genetic characterization of sialidase from a wild isolate. Sialidase, which has undergone further production and purification processes exposed to chicken embryonic fibroblast cell culture, and observations-based structural morphology of cells compared between treated cells and normal cells without treatment.

Potency of bacterial sialidase as antiviral of Newcastle disease infections using embryonated chicken egg model.

Background And Aim: toxins are widely used as medicinal agents. Many active metabolic enzymes, including sialidase (neuraminidase), hyaluronidase, and collagenase, contribute to the mechanism of action of these toxins. Sialidase from recognizes and degrades sialic acid receptors in the host cell glycoprotein, glycolipid, and polysaccharide complexes.

Evaluation of inhibitor activity of bacterial sialidase from against Newcastle disease virus in the cell culture model using chicken embryo fibroblast.

Objective: The Newcastle disease virus (NDV) is an infectious disease that causes very high economic losses due to decreased livestock production and poultry deaths. The vaccine's ineffectiveness due to mutation of the genetic structure of the virus impacts obstacles in controlling the disease, especially in some endemic areas. This study aimed to provide an alternative treatment for NDV infection by observing the viral replication inhibitor activity of sialidase in primary chicken embryo fibroblast (CEF) cells.

Screening and purification of NanB sialidase from Pasteurella multocida with activity in hydrolyzing sialic acid Neu5Acα(2-6)Gal and Neu5Acα(2-3)Gal.

Study on sialidases as antiviral agents has been widely performed, but many types of sialidase have not been tested for their antiviral activity. Pasteurella multocida NanB sialidase is one such sialidase that has never been isolated for further research. In this study, the activity of NanB sialidase was investigated in silico by docking the NanB sialidase of Pasteurella multocida to the Neu5Acα(2-6)Gal and Neu5Acα(2-3)Gal ligands.

Isolation and molecular characterization of the gene of H9N2 avian influenza viruses from poultry in Java, Indonesia.

Objective: The avian influenza virus (AIV) subtype H9N2 circulating in Indonesia has raised increasing concern about its impact on poultry and its public health risks. In this study, the H9N2 virus from chicken poultry farms in Java was isolated and characterized molecularly.

Materials And Methods: Thirty-three pooled samples of chicken brain, cloacal swab, trachea, and oviduct were taken from multiple chickens infected with AIV in five regions of Java, Indonesia.

Molecular detection of genes encoding resistance to tetracycline and determination of plasmid-mediated resistance to quinolones in avian pathogenic in Sukabumi, Indonesia.

Aim: This study aimed to identify genes encoding resistance to tetracycline (TE) and plasmid-mediated resistance to quinolones in isolates from clinical cases of avian colibacillosis in Sukabumi, Indonesia.

Materials And Methods: A total of 25 archive isolates were collected in 2013-2017 from clinical cases of avian colibacillosis in Sukabumi, Indonesia. All isolates were tested for TE and quinolone resistance using the disk diffusion method.