Vehicle re-identification is a demanding and challenging task in automated surveillance systems. The goal of vehicle re-identification is to associate images of the same vehicle to identify re-occurrences of the same vehicle. Robust re-identification of individual vehicles requires reliable and discriminative features extracted from specific parts of the vehicle.
View Article and Find Full Text PDFIn this work, a framework is proposed for decision fusion utilizing features extracted from vehicle images and their detected wheels. Siamese networks are exploited to extract key signatures from pairs of vehicle images. Our approach then examines the extent of reliance between signatures generated from vehicle images to robustly integrate different similarity scores and provide a more informed decision for vehicle matching.
View Article and Find Full Text PDFThe authors regret that they neglected to cite their conference report on the technical part of a 'preliminary study' presented at, and published in, the Biomedical Sciences and Engineering Conference (BSEC), 2010, May 25-26 (Fully automated segmentation and characterization of the dendritic trees of retinal horizontal neurons -DOI: 10.1109/BSEC.2010.
View Article and Find Full Text PDFCircuits in the auditory cortex are highly susceptible to acoustic influences during an early postnatal critical period. The auditory cortex selectively expands neural representations of enriched acoustic stimuli, a process important for human language acquisition. Adults lack this plasticity.
View Article and Find Full Text PDFNeuronal migration from a germinal zone to a final laminar position is essential for the morphogenesis of neuronal circuits. While it is hypothesized that microtubule-actomyosin crosstalk is required for a neuron's 'two-stroke' nucleokinesis cycle, the molecular mechanisms controlling such crosstalk are not defined. By using the drebrin microtubule-actin crosslinking protein as an entry point into the cerebellar granule neuron system in combination with super-resolution microscopy, we investigate how these cytoskeletal systems interface during migration.
View Article and Find Full Text PDFBackground: During brain development, neurons migrate from germinal zones to their final positions to assemble neural circuits. A unique saltatory cadence involving cyclical organelle movement (e.g.
View Article and Find Full Text PDFIn this paper, we study segmentation of tight junctions and analyze the formation and integrity of tight junctions in large-scale confocal image stacks, a challenging biological problem because of the low spatial resolution images and the presence of breaks in tight junction structure. We present an automated, three-step processing approach for tight junction analysis. In our approach, we first localize each individual nucleus in the image by using thresholding, morphological filters and active contours.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
December 2011
Neuronal differentiation with respect to the acquisition of synaptic competence needs to be regulated precisely during neurogenesis to ensure proper formation of circuits at the right place and time in development. This regulation is particularly important for synaptic triads among photoreceptors, horizontal cells (HCs), and bipolar cells in the retina, because HCs are among the first cell types produced during development, and bipolar cells are among the last. HCs undergo a dramatic transition from vertically oriented neurites that form columnar arbors to overlapping laminar dendritic arbors with differentiation.
View Article and Find Full Text PDFIn the developing mammalian retina, horizontal neurons undergo a dramatic reorganization of their processes shortly after they migrate to their appropriate laminar position. This is an important process because it is now understood that the apical processes are important for establishing the regular mosaic of horizontal cells in the retina and proper reorganization during lamination is required for synaptogenesis with photoreceptors and bipolar neurons. However, this process is difficult to study because the analysis of horizontal neuron anatomy is labor intensive and time-consuming.
View Article and Find Full Text PDFAnnu Int Conf IEEE Eng Med Biol Soc
April 2010
In order to facilitate the study of neuron migration, we propose a method for 3-D detection and tracking of centrosomes in time-lapse confocal image stacks of live neuron cells. We combine Laplacian-based blob detection, adaptive thresholding, and the extraction of scale and roundness features to find centrosome-like objects in each frame. We link these detections using the joint probabilistic data association filter (JPDAF) tracking algorithm with a Newtonian state-space model tailored to the motion characteristics of centrosomes in live neurons.
View Article and Find Full Text PDFLamination of cortical regions of the vertebrate brain depends on glial-guided neuronal migration. The conserved polarity protein Par6alpha localizes to the centrosome and coordinates forward movement of the centrosome and soma in migrating neurons. The cytoskeletal components that produce this unique form of cell polarity and their relationship to polarity signaling cascades are unknown.
View Article and Find Full Text PDFCorrelation filtering methods are becoming increasingly popular for image recognition and location. The recent introduction of optimal tradeoff circular harmonic function filters allowed the user to specify the response of a correlation filter to in-plane rotation distortion. In this paper we introduce a new correlation filter design that can provide a user-specified response to in-plane scale distortion.
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