Measurement of functional fibrinogen levels using the Thrombelastograph.

Study Objective: To validate a Thromboelastograph (Haemoscope Corporation, Niles, IL) assay for functional fibrinogen.

Design: Correlation study of the Thromboelastograph assay with two conventional fibrinogen assays by the standard Clauss method.

Setting: Research laboratory of a university medical center.

Thrombelastography monitoring of resistance to enoxaparin anticoagulation in thrombophilic pregnancy patients.

The anticoagulant effect of enoxaparin is readily observed by Thrombelastography (TEG), particularly on the reaction time (R) to form a clot, and is completely reversed by heparinase. In this study, recalcified citrated whole blood with heparinase (CNHR) and without (CNR), along with TEG R time, was used to derive a delta R (CNR-CNHR). This delta R (DeltaR) was then used to measure enoxaparin anticoagulation, which was correlated by linear regression (r(2)=0.