Examining the proteins of functional retinal lipofuscin using proteomic analysis as a guide for understanding its origin.

Purpose: To elucidate the origins of biologically active retinal lipofuscin (RLF) by examining its protein composition.

Methods: Total protein and total lipid were extracted and quantified. Proteins in this lipoprotein granule were identified by limited-scale proteomic analysis using both two-dimensional (2D) gel electrophoresis and SDS-PAGE coupled with MALDI-QqToF MSMS and automated LCMSMS, respectively.