Styrene-Maleic Acid Copolymer Nanodiscs to Determine the Shape of Membrane Proteins.

Lipid nanodiscs can be used to solubilize functional membrane proteins (MPs) in nativelike environments. Thus, they are promising reagents that have been proven useful to characterize MPs. Both protein and non-protein molecular belts have shown promise to maintain the structural integrity of MPs in lipid nanodiscs.

Kinetic CRAC uncovers a role for Nab3 in determining gene expression profiles during stress.

RNA-binding proteins play a key role in shaping gene expression profiles during stress, however, little is known about the dynamic nature of these interactions and how this influences the kinetics of gene expression. To address this, we developed kinetic cross-linking and analysis of cDNAs (χCRAC), an ultraviolet cross-linking method that enabled us to quantitatively measure the dynamics of protein-RNA interactions in vivo on a minute time-scale. Here, using χCRAC we measure the global RNA-binding dynamics of the yeast transcription termination factor Nab3 in response to glucose starvation.

Load-induced stresses in photoelastic primary canines with facial restorations.

Purpose: The purpose of this study was to photoelastically compare the stresses generated by loads on primary canines with facial restorations of different stiffness.

Methods: Composite photoelastic models of a typical maxillary primary canine were fabricated using individual simulant materials for enamel, dentin, periodontal ligament, and alveolar bone. Models were made with identical facial preparations included either near the cementoenamel junction or at 2 mm incisally.