Controlled atmosphere treatment of broccoli after harvest delays senescence and induces the expression of novel BoCAR genes.

The current study examines the transcription of four genes (BoCAR1A, BoCAR5, BoCAR6-4 and BoCAR25) found to be up-regulated in response to high CO(2)/low O(2) treatment in broccoli (Brassica oleracea). Messenger RNA levels for the four genes declined after tissues were removed from CA. Physiological and biochemical changes and gene expression patterns were examined in broccoli tissues held in one of four different atmospheres, namely air (<1% CO(2), 21% O(2)), high carbon dioxide and low oxygen (CA 10% CO(2), 5% O(2)), low oxygen (0% CO(2), 5% O(2)), and high carbon dioxide (10% CO(2), 20% O(2)).

Transcriptional profiling of human cord blood CD133+ and cultured bone marrow mesenchymal stem cells in response to hypoxia.

Umbilical cord blood (UCB) and bone marrow (BM)-derived stem and progenitor cells possess two characteristics required for successful tissue regeneration: extensive proliferative capacity and the ability to differentiate into multiple cell lineages. Within the normal BM and in pathological conditions, areas of hypoxia may have a role in maintaining stem cell fate or determining the fine equilibrium between their proliferation and differentiation. In this study, the transcriptional profiles and proliferation and differentiation potential of UCB CD133(+) cells and BM mesenchymal cells (BMMC) exposed to normoxia and hypoxia were analyzed and compared.

Galactosidases in opening, senescing and water-stressed Sandersonia aurantiaca flowers.

Three glycosyl hydrolase family 35 β-galactosidase-encoding cDNAs, SaGAL1 (full-length), SaGAL2 and SaGA3L (both partial), have been isolated from Sandersonia aurantiaca (Hook.) SaGAL1 protein was functionally expressed in E. coli and β-galactosidase identity confirmed by activity assay.

Distinct cis-elements in the Asparagus officinalis asparagine synthetase promoter respond to carbohydrate and senescence signals.

The Asparagus officinalis L. asparagine (Asn) synthetase (AS) promoter was analysed for elements responding to carbohydrate and senescence signals. Transgenic Arabidopsis thaliana L.

Molecular analysis of programmed cell death during senescence in Arabidopsis thaliana and Brassica oleracea: cloning broccoli LSD1, Bax inhibitor and serine palmitoyltransferase homologues.

This study was undertaken to characterize the programmed cell death (PCD) processes that occur during detached and natural on-plant senescence and correlate them with the expression of putative regulatory genes that may be involved in the process. DNA fragmentation and TUNEL analysis of broccoli florets showed that DNA was processed into fragments of approximately 180 bp after 48 h of harvest-induced tissue senescence. Characteristic laddering patterns were also visible in Arabidopsis leaves undergoing natural on-plant senescence and during detached senescence.

Programmed cell death during flower senescence: isolation and characterization of cysteine proteinases from Sandersonia aurantiaca.

Cysteine protease inhibitors delayed the senescence of Sandersonia aurantiaca Hook. flowers. Tepal fading and wilting occurred later in the 2,2´ -dipyridyl-treated flowers, and these flowers had a greater soluble protein content and less active endoproteases compared with control flowers that were held in water.