Publications by authors named "Ruyue Ji"
Article Synopsis
- - Aspartic acid (Asp) isomerization is a non-enzymatic change that occurs in proteins, especially therapeutic antibodies, during manufacturing and storage, affecting their structure and function.
- - Certain Asp motifs like Asp-Gly, Asp-Ser, and Asp-Thr are known as "hot spots" for isomerization, while Asp-His is typically a "silent spot" until this study found high isomerization in the Asp residue of the DHK motif in monoclonal antibody mAb-a.
- - The study revealed that isomerization in the DHK motif can reduce antibody activity significantly, despite not affecting pharmacokinetics; therefore, it’s recommended to remove DHK motifs from antibody therapeutics
The EP1 prostanoid receptor is one of four subtypes whose cognate physiological ligand is prostaglandin-E2 (PGE(2)). It is in the family of G-protein-coupled receptors and is known to activate Ca(2+) signaling, although relatively little is known about other aspects of E-type prostanoid receptor (EP) 1 receptor signaling. In human embryonic kidney (HEK) cells expressing human EP1 receptors, we now show that PGE(2) stimulation of the EP1 receptor up-regulates the expression of hypoxia-inducible factor-1 alpha (HIF-1 alpha), which can be completely blocked by pertussis toxin, indicating coupling to G(i/o).
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