Isolation and identification of triterpenoid saponins with antiproliferative and hemolytic activities from Camellia oleifera Abel seeds.

Seven previously undescribed triterpenoid saponins, oleiferasaponins G-G (1-7), along with two known congeners (8 and 9) were isolated from seeds of Camellia oleifera Abel. Their structures were determined by extensive spectroscopic data. All isolated compounds are decorated with an aglycone and tetrasaccharide moiety.

The chemical basis of aroma/taste and color formation in green tea infusion during cold brewing revealed by metabolomics analysis.

In this study, metabolomics and chemometrics were utilized to comprehensively investigate chemical mechanisms of aroma, taste, and color formation in cold-brewed green tea (4 °C). The results showed that the typical flavor of cold-brewed green tea (tea-to-water ratio: 1:50 g/mL) developed gradually after 1 h. Compared with the hot-brewed (80 °C) condition, volatile alcohols accumulated more under cold-brewing conditions.

A "three-in-one" detection platform based on cerium-coated metal-organic framework for multimodal glyphosate detection.

Glyphosate (Glyp) is a widely used herbicide, but its through prolonged and excessive spraying residues pose significant health risks. A ratiometric multimodal sensing platform combining fluorescence, colorimetry, and photothermal detection was developed for Glyp detection using a cerium/polyacrylic acid-modified porphyrin-based metal-organic framework (PCN@PAA-Ce). The detection strategy relies on the inhibition of acetylcholinesterase by Glyp, the oxidase-like activity of PCN@PAA-Ce, and the competition between enzymatic products and 3,3',5,5'-tetramethylbenzidine (TMB).

Two New Triterpenoid Saponins with Antifungal Activity from Flowers.

Two new triterpenoid saponins, namely camsinsaponins A and B (, ), along with two known congeners (, ) were isolated from flowers. Their structures were determined by extensive spectroscopic data. All compounds were assessed for antifungal bioactivity against , , and .

Towards healthier low-sugar and low-fat beverages: Design, production, and characterization.

Many consumers are adopting low-sugar and low-fat beverages to avoid excessive calories and the negative impact of high trans- and/or saturated fat on health and wellbeing. This article reviews strategies to reduce sugar, fat, and high trans- and/or saturated fat content in beverages while maintaining their desirable physicochemical and sensory attributes. It assesses the impact of various sugar and fat replacers on the aroma, taste, texture, appearance, and nutritional profile of beverages.

Application of lanthanum-modified silk fibroin/polyvinyl alcohol film for highly selective defluoridation in brick tea infusion.

To mitigate the risk associated with water-soluble fluoride in tea and to have less influence on the contents of tea infusion, a highly selective lanthanum modified silk fibroin (SF) and polyvinyl alcohol (PVA) composite film (SF/PVA-La) was prepared to remove fluoride from brick tea infusion. Notably, SF/PVA-La could remove about 48 % of the fluoride from in brick tea infusion within 30 min. Importantly, the reduction in total tea polyphenols in brick tea did not exceed 10 %, and the reduction in caffeine was only 0.

Real-Time In Situ Tracking of the Penetration and Uptake Behavior of Nano-pesticides in Tea Plants Using Surface-Enhanced Raman Spectroscopy.

In situ monitoring of the uptake and transportation process of nano-pesticides during crop growth remains challenging thus far. In this report, the different three sizes of surface-enhanced Raman spectroscopy probes of nano-pesticides loaded with ferbam and acetamiprid are representative non-systemic or systemic pesticides, respectively. The probes were verified to have strong signals of the Raman spectrum enhancement effect.

Design strategies of polysaccharide, protein and lipid-based nano-delivery systems in improving the bioavailability of polyphenols and regulating gut homeostasis.

Polyphenols are plant secondary metabolites that have attracted much attention due to their anti-inflammatory, antioxidant, and gut homeostasis promoting effects. However, food matrix interaction, poor solubility, and strong digestion and metabolism of polyphenols cause barriers to their absorption in the gastrointestinal tract, which further reduces bioavailability and limits polyphenols' application in the food industry. Nano-delivery systems composed of biocompatible macromolecules (polysaccharides, proteins and lipids) are an effective way to improve the bioavailability of polyphenols.

Lateral Flow Immunoassay for the Rapid Detection of Thiamethoxam in Tea Based on a SERS Tag Constructed by Phenolic-Mediated Coating Engineering.

Sensitive and accurate detection of thiamethoxam in tea is significant to ensuring consumer health. In this study, surface-enhanced Raman scattering (SERS) tags were prepared by using a polyphenol-mediated coating engineering strategy. This approach involved the self-assembly of tannic acid (TA) and self-polymerization of benzene-1,4-dithiol (BDT) on the surface of gold nanoparticles, resulting in the formation of Au@pBDT-TA.

Tea's Characteristic Components Eliminate Acrylamide in the Maillard Model System.

This study investigated the effects of various characteristic components of tea-theaflavins, catechins, thearubigins, theasinensins, theanine, catechin (C), catechin gallate (CG), epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC), epigallocatechin gallate (EGCG), gallocatechin (GC), and gallocatechin gallate (GCG)-on acrylamide formation. The results revealed that most of tea's characteristic components could significantly eliminate acrylamide, ranked from highest to lowest as follows: GC (55.73%) > EC (46.

A novel fluorescent and photothermal probe based on nanozyme-mediated cascade reaction for detecting organophosphorus pesticide residues.

In this study, a nanozyme (ZIF-Co-Cys) with high oxidase-like catalytic activity was prepared, and a ratiometric fluorescent/photothermal dual-mode probe was constructed for organophosphorus pesticides (OPs) detection based on the competitive effect of ZIF-Co-Cys and the enzymatic reaction product of acid phosphatase (ACP) on o-phenylenediamine and the inhibition effect of OPs on ACP activity. Using dimethyl dichloroviny phosphate (DDVP) as the model, both the fluorescence intensity ratio and the temperature change of the probe solution exhibited an excellent correlation with OPs concentration. The detection limits were 1.

Rapid, in-situ evaluation of sunflower seed freshness and vigor using Raman microspectroscopy scanning of carotenoids.

An ultra-rapid, in-situ Raman microscopy strategy was developed for judging both seed freshness and seed vigor based on relative quantification of carotenoids content during sunflower seed germination. The carotenoids content was determined using the ratio of the Raman peak intensities at 1525 and 1268 cm (I). When different samples (harvest times and storage conditions) were soaked in water for 0-24 h, the carotenoids content in the embryonic axes gradually increased, with the carotenoids higher in fresher seeds.

Zirconium‑cerium modified polyvinyl alcohol/NaCMC biocomposite film: Synthesis of films through high-speed shear assisted technique and removal fluoride from water.

A new zirconium and cerium-modified polyvinyl alcohol (PVA) sodium carboxymethyl cellulose (NaCMC) film (PVA/CMC-Zr-Ce) was synthesized thru a high-speed shear-assisted method and its adsorption for the removal of fluoride was studied, in which the NaCMC provided -COONa for ion exchange between Na and Zr-Ce, thus the loading amount of Zr-Ce on films was accordingly increased. The morphology and structure of PVA/CMC-Zr-Ce were characterized using scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and X-ray diffraction (XRD). Besides, the mechanical properties, water contact angle, and swelling ratio of film were also evaluated.

Dietary polyphenols regulate appetite mechanism via gut-brain axis and gut homeostasis.

Nowadays, due to the rise of fast-food consumption, the metabolic diseases are increasing as a result of high-sugar and high-fat diets. Therefore, there is an urgent need for natural, healthy and side-effect-free diets in daily life. Whole grain supplementation can enhance satiety and regulate energy metabolism, effects that have been attributed to polyphenol content.

Mn-modified porphyrin metal-organic framework mediated colorimetric and photothermal dual-channel probe for sensitive detection of organophosphorus pesticides.

Herein, a novel dual-mode probe for organophosphorus pesticides (OPs) colorimetric and photothermal detection was developed based on manganese modified porphyrin metal-organic framework (PCN-224-Mn). PCN-224-Mn had excellent oxidase-like activity and oxidized colorless 3,3,5,5-tetramethylbenzidine (TMB) to blue-green oxidation state TMB (oxTMB), which exhibited high temperature under near-infrared irradiation. l-ascorbate-2-phosphate was hydrolyzed by acid phosphatase to produce ascorbic acid, which weakened colorimetric and photothermal signals by impacting oxTMB generation.

A facile fluorescence microplate immunoassay based on an in situ fluorogenic reaction for the detection of two highly toxic anticoagulant rodenticides in food and biological matrix.

Bromadiolone and brodifacoum, the most frequently used anticoagulant rodenticides, are highly toxic and pose a threat to public health by causing food poisoning incidents. Here, we developed a fluorescence microplate immunoassay for facile and sensitive detection of bromadiolone and brodifacoum by introducing three commercial chemicals (p-phenylenediamine, polyethyleneimine, HO) as a new substrate of horseradish peroxidase and then generating fluorescence signals based on an in situ fluorogenic reaction (detection time within 75 min). This assay exhibited higher efficiency in generating fluorescence signals, thereby exhibiting a 6-fold improvement in sensitivity compared with colorimetric ELISA.

Selenium foliar application contributes to decrease ratio of water-soluble fluoride and improve physio-biochemical components in tea leaves.

The tea plant accumulates elevated levels of fluoride (F) from environmental sources. Drinking tea containing high F levels poses a potential threat to human health. Selenium (Se) was applied by foliar spray to investigate its effects on F accumulation and physiology in tea plant.

Capability of phytoremediation of glyphosate in environment by Vulpia myuros.

Glyphosate is an herbicide extensively used worldwide that can remain in the soil. Phytoremediation to decontaminate polluted water or soil requires a plant that can accumulate the target compound. Vulpia myuros is an annual fescue that can be used as a heavy mental phytoremediation strategy.

Enzyme-mediated Ru@UiO-66@MnO NSs/thiamine-based ratiometric fluorescence sensor for visual detection of organophosphorus pesticide residues.

In view of the potential hazards of organophosphorus pesticides (OPs), this paper constructed a ratiometric fluorescent probe utilizing a functionalized metal-organic framework to detect OPs. Ru(bpy)Cl was encapsulated inside UiO-66 as a reference signal, and MnO nanosheets (MnO NSs) were grown on the surface to obtain Ru@UiO-66@MnO NSs. Acetylcholinesterase catalyzed the decomposition of acetylcholine into reductive thiocholine, which consumed MnO NSs, thus restoring the Ru@UiO-66 fluorescence.

Lipidomic profiling study on neurobehavior toxicity in zebrafish treated with aflatoxin B1.

Mycotoxin aflatoxin B1 (AFB1) has been proven to cause neurotoxicity, but its potential interference with the normal function of brain tissue is not fully defined. As the indispensable role of lipids in maintaining the normal function of brain tissue, the aim of this study is to clarify the effect of AFB1 short-term (7 days) exposure on brain tissue from the perspective of lipid metabolism. In this study, zebrafish were exposed to two concentrations (5, 20 μg/L).

Simultaneous Analysis of 53 Pesticides in Safflower ( L.) by Using LC-MS/MS Coupled with a Modified QuEChERS Technique.

Objective: An optimized quick, easy, cheap, effective, rugged, and safe (QuEChERS) technique was investigated and compared with the conventional QuEChERS technique for the simultaneous analysis of fifty-three pesticide residues in safflower using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS).

Method: Graphitic carbon nitride (g-CN) consisting of a major amount of carbon and nitrogen with a large surface area was used as a QuEChERS adsorbent instead of graphitized carbon black (GCB) for safflower extraction purification. Validation experiments were performed using spiked pesticide samples, and real samples were analyzed.